Zhao Yingying, Li Qin, Hu Xiaoming, Lo Yuhwa
School of Life Science, Key Laboratory of Convergence Medical Engineering System and Healthcare Technology, Beijing Institute of Technology , Beijing 100081, China.
Department of Electrical and Computer Engineering, University of California San Diego , California 92093-0407, USA.
Biomicrofluidics. 2016 Dec 23;10(6):064119. doi: 10.1063/1.4972105. eCollection 2016 Nov.
A microfluidic cytometer with integrated on-chip optical systems was designed for red blood cell (RBC) and platelet (PLT) counting. The design, fabrication, and characterization of the microfluidic cytometer with on-chip optical signal detection were described. With process using only a single mask, the device that integrates optical fibers and on-chip microlens with microfluidic channels on a polydimethylsiloxane layer by standard soft photolithography. This compact structure increased the sensitivity of the device and eliminated time-consuming free-space optical alignments. The microfluidic cytometer was used to count red blood cells and platelets. Forward scatter and extinction were collected simultaneously for each cell. Experimental results indicated that the microfluidic cytometer exhibited comparable performance with a conventional cytometer and demonstrated superior capacity to detect on-chip optical signals in a highly compact, simple, truly portable, and low-cost format that is well suitable for point-of-care clinical diagnostics.
一种集成了片上光学系统的微流控血细胞计数器被设计用于红细胞（RBC）和血小板（PLT）计数。描述了具有片上光信号检测功能的微流控血细胞计数器的设计、制造和特性。通过仅使用单个掩膜的工艺，该设备通过标准软光刻技术在聚二甲基硅氧烷层上集成了光纤、片上微透镜和微流控通道。这种紧凑的结构提高了设备的灵敏度，并消除了耗时的自由空间光学对准。该微流控血细胞计数器用于计数红细胞和血小板。同时收集每个细胞的前向散射和消光信号。实验结果表明，该微流控血细胞计数器与传统血细胞计数器具有相当的性能，并展示了以高度紧凑、简单、真正便携且低成本的形式检测片上光信号的卓越能力，非常适合即时医疗临床诊断。
