Cavalli Roberta, Donalisio Manuela, Bisazza Agnese, Civra Andrea, Ranucci Elisabetta, Ferruti Paolo, Lembo David
Dipartimento di Scienza e Tecnologia del Farmaco, Università di Torino, Torino, Italy.
Methods Enzymol. 2012;509:1-19. doi: 10.1016/B978-0-12-391858-1.00001-0.
The activity of antivirals can be enhanced by their incorporation in nanoparticulate delivery systems. Peculiar polymeric nanoparticles, based on a β-cyclodextrin-poly(4-acryloylmorpholine) monoconjugate (β-CD-PACM), are proposed as acyclovir carriers. The experimental procedure necessary to obtain the acyclovir-loaded nanoparticles using the solvent displacement preparation method will be described in this chapter. Fluorescent labeled nanoparticles are prepared using the same method for cellular trafficking studies. The biocompatibility assays necessary to obtain safe nanoparticles are reported. Section 4 of this chapter describes the assessment of the antiviral activity of the acyclovir-loaded nanoparticles.
抗病毒药物的活性可通过将其纳入纳米颗粒递送系统而得到增强。基于β-环糊精-聚（4-丙烯酰基吗啉）单共轭物（β-CD-PACM）的特殊聚合物纳米颗粒被提议作为阿昔洛韦的载体。本章将描述使用溶剂置换制备方法获得负载阿昔洛韦纳米颗粒所需的实验步骤。使用相同方法制备荧光标记纳米颗粒用于细胞转运研究。报告了获得安全纳米颗粒所需的生物相容性测定。本章第4节描述了负载阿昔洛韦纳米颗粒抗病毒活性的评估。
