大鼠甲状腺素运载蛋白基因的双启动子与组织特异性表达

Dual promoters and tissue-specific expression of rat transthyretin gene.

作者信息

Motojima K, Goto S

机构信息

Department of Biochemistry, School of Pharmaceutical Sciences, Toho University, Chiba, Japan.

出版信息

Biochem Biophys Res Commun. 1990 Nov 30;173(1):323-30. doi: 10.1016/s0006-291x(05)81060-5.

DOI:10.1016/s0006-291x(05)81060-5

PMID:2256922

Abstract

The rat chromosomal gene for transthyretin was cloned and its dual promoter region was analyzed by primer extension. Semi-quantitative studies by primer extension analysis to determine the transcriptional start sites and their usage in the three tissues have suggested that the rat transthyetin gene has dual promoters: a major proximal promoter which is used in the liver and in the brain but not at all in the kidney, and a minor distal promoter which is totally used in the kidney and significantly in the brain.

摘要

克隆了大鼠甲状腺素运载蛋白的染色体基因，并通过引物延伸分析其双启动子区域。通过引物延伸分析进行的半定量研究，以确定三种组织中转录起始位点及其使用情况，结果表明大鼠甲状腺素运载蛋白基因有双启动子：一个主要的近端启动子，在肝脏和大脑中使用，但在肾脏中完全不使用；一个次要的远端启动子，在肾脏中完全使用，在大脑中也大量使用。