Jang Sujeong, Park Jong-Sung, Won Young-Ho, Yun Sook-Jung, Kim Seong-Jin
Department of Physiology, Chonnam National University Medical School, Gwangju, Korea.
Chonnam Med J. 2012 Apr;48(1):7-14. doi: 10.4068/cmj.2012.48.1.7. Epub 2012 Apr 26.
Fibroblasts are responsible for the synthesis and degradation of various connective tissue components and soluble mediators of extracellular matrix metabolism. Few studies have been conducted concerning the expression of toll-like receptors (TLRs) in fibroblasts until now. This study aimed first to determine the quantitative expression of TLRs 1 to 10 in human skin fibroblasts and secondarily to explore any influence of expression by histamine, which is a well-known factor engaged in dermal inflammation. It was found that all 10 TLRs were expressed in fibroblasts. Interestingly, the expression of TLRs 4, 5, and 10 was increased after 2 and 6 hours of histamine treatment during culture. However, the expression of TLRs 2, 3, 6, 7, 8, and 9 was decreased after 6 hours of histamine treatment. Among the TLRs with a decreasing expression pattern, TLRs 7 and 8 showed a persistent tendency to decrease. All of these changes in TLR expression with histamine treatment were antagonized by treatment with diphenhydramine, a well-known antihistamine. Thus, these results suggest a role of histamine in the early phase of the dermal inflammatory reaction mediated by TLRs.
成纤维细胞负责合成和降解各种结缔组织成分以及细胞外基质代谢的可溶性介质。到目前为止，关于成纤维细胞中Toll样受体（TLRs）的表达研究较少。本研究旨在首先确定人皮肤成纤维细胞中TLR1至TLR10的定量表达，其次探讨组胺（一种参与皮肤炎症的知名因子）对其表达的影响。研究发现，所有10种TLR均在成纤维细胞中表达。有趣的是，在培养过程中组胺处理2小时和6小时后，TLR4、TLR5和TLR10的表达增加。然而，组胺处理6小时后，TLR2、TLR3、TLR6、TLR7、TLR8和TLR9的表达降低。在表达呈下降模式的TLR中，TLR7和TLR8呈现持续下降趋势。所有这些组胺处理引起的TLR表达变化均被知名抗组胺药苯海拉明处理所拮抗。因此，这些结果表明组胺在由TLRs介导的皮肤炎症反应早期发挥作用。
