Department of Medical Technology, Anhui Medical College, First affiliated hospital of Anhui Medical University, Hefei, China.
J Pharm Pharmacol. 2012 Jun;64(6):882-7. doi: 10.1111/j.2042-7158.2012.01480.x. Epub 2012 Mar 27.
Bidens bipinnata L. is well known as a traditional antipyretic, anti-inflammatory and anti-rheumatic medicine in China. This study was designed to evaluate the role of total extracted flavonoids from B. bipinnata (TFB) in inhibiting the production of inflammatory cytokines.
Human umbilical vein endothelial cells (HUVEC) were used to examine the effect of TFB on the production of inflammatory cytokines. The supernatant interleukin (IL)-8, tumour necrosis factor (TNF)-α and nitric oxide (NO) levels of HUVEC were measured with ELISA methods. Nuclear factor-kappaB (NF-κB) and fractalkine expression was evaluated by RT-PCR and Western blot methods, respectively.
We observed that IL-8, TNF-α and NO release of HUVEC incubated with sera from active Henoch-Schönlein purpura (HSP) was significantly increased. TFB intervention may significantly suppressed the supernatant IL-8, TNF-α and NO levels of HUVEC. Similarly, TFB obviously suppressed the NF-κB and fractalkine mRNA and protein expression.
These results suggested that TFB may be useful for improving microvascular inflammation in HSP patients.
双蝴蝶（Bidens bipinnata L.）在中国是一种众所周知的传统解热、抗炎和抗风湿药物。本研究旨在评估双蝴蝶总提黄酮（TFB）在抑制炎症细胞因子产生中的作用。
用人脐静脉内皮细胞（HUVEC）检测 TFB 对炎症细胞因子产生的影响。采用 ELISA 法测定 HUVEC 上清液中白细胞介素（IL）-8、肿瘤坏死因子（TNF）-α和一氧化氮（NO）水平。分别采用 RT-PCR 和 Western blot 法评估核因子-κB（NF-κB）和 fractalkine 的表达。
我们观察到，与来自活动期过敏性紫癜（HSP）患者的血清孵育的 HUVEC 的 IL-8、TNF-α和 NO 释放明显增加。TFB 干预可能显著抑制 HUVEC 上清液中 IL-8、TNF-α和 NO 的水平。同样，TFB 明显抑制了 NF-κB 和 fractalkine 的 mRNA 和蛋白表达。
这些结果表明，TFB 可能有助于改善 HSP 患者的微血管炎症。
