Program in Biomedical Sciences and Engineering, Department of Molecular, Cell, and Developmental Biology, University of California-Santa Cruz, Santa Cruz, CA, 95064, USA.
Department of Public Health, Experimental and Forensic Medicine, Histology and Embryology Unit, University of Pavia, Pavia, Italy.
Stem Cell Rev Rep. 2022 Apr;18(4):1294-1308. doi: 10.1007/s12015-021-10326-4. Epub 2022 Jan 11.
One of the most exciting advances in life science research is the development of 3D cell culture systems to obtain complex structures called organoids and spheroids. These 3D cultures closely mimic in vivo conditions, where cells can grow and interact with their surroundings. This allows us to better study the spatio-temporal dynamics of organogenesis and organ function. Furthermore, physiologically relevant organoids cultures can be used for basic research, medical research, and drug discovery. Although most of the research thus far focuses on the development of heart, liver, kidney, and brain organoids, to name a few, most recently, these structures were obtained using dental stem cells to study in vitro tooth regeneration. This review aims to present the most up-to-date research showing how dental stem cells can be grown on specific biomaterials to induce their differentiation in 3D. The possibility of combining engineering and biology principles to replicate and/or increase tissue function has been an emerging and exciting field in medicine. The use of this methodology in dentistry has already yielded many interesting results paving the way for the improvement of dental care and successful therapies.
生命科学研究中最令人兴奋的进展之一是开发 3D 细胞培养系统，以获得称为类器官和球体的复杂结构。这些 3D 培养物非常类似于体内条件，细胞可以在其中生长并与其周围环境相互作用。这使我们能够更好地研究器官发生和器官功能的时空动态。此外，生理相关的类器官培养物可用于基础研究、医学研究和药物发现。尽管迄今为止大多数研究都集中在心脏、肝脏、肾脏和大脑类器官等的开发上，但最近，这些结构是使用牙齿干细胞在体外获得的，用于研究牙齿再生。本综述旨在介绍最新的研究，展示如何在特定生物材料上培养牙齿干细胞以诱导其在 3D 中的分化。将工程学和生物学原理相结合以复制和/或增加组织功能的可能性一直是医学领域中一个新兴且令人兴奋的领域。该方法在牙科中的应用已经产生了许多有趣的结果，为改善牙科护理和成功治疗铺平了道路。
