Fusion Biotechnology Research Center, KRICT, Daejeon 305-600, Republic of Korea; E-Mails:
Sensors (Basel). 2009;9(4):2334-44. doi: 10.3390/s90402334. Epub 2009 Mar 30.
To amplify the difference in localized surface plasmon resonance (LSPR) spectra of gold nano-islands due to intermolecular binding events, gold nanoparticles were used. LSPR-based optical biosensors consisting of gold nano-islands were readily made on glass substrates using evaporation and heat treatment. Streptavidin (STA) and biotinylated bovine serum albumin (Bio-BSA) were chosen as the model receptor and the model analyte, respectively, to demonstrate the effectiveness of this detection method. Using this model system, we were able to enhance the sensitivity in monitoring the binding of Bio-BSA to gold nano-island surfaces functionalized with STA through the addition of gold nanoparticle-STA conjugates. In addition, SU-8 well chips with gold nano-island surfaces were fabricated through a conventional UV patterning method and were then utilized for image detection using the attenuated total reflection mode. These results suggest that the gold nano-island well chip may have the potential to be used for multiple and simultaneous detection of various bio-substances.
为了放大金纳米岛之间由于分子间结合事件而导致的局域表面等离子体共振（LSPR）光谱的差异，使用了金纳米粒子。通过蒸发和热处理，很容易在玻璃衬底上制作基于 LSPR 的光学生物传感器，其中包含金纳米岛。链霉亲和素（STA）和生物素化牛血清白蛋白（Bio-BSA）分别被选为模型受体和模型分析物，以证明这种检测方法的有效性。使用这个模型系统，我们能够通过添加金纳米粒子-STA 缀合物，增强监测 Bio-BSA 与 STA 功能化的金纳米岛表面结合的灵敏度。此外，通过传统的 UV 图案化方法制造了具有金纳米岛表面的 SU-8 井芯片，然后利用衰减全反射模式进行图像检测。这些结果表明，金纳米岛井芯片可能具有用于多种和同时检测各种生物物质的潜力。
