Das Sonatan, Agarwal Dilip Kumar, Mandal Bikash, Rao V Ramgopal, Kundu Tapanendu
Centre for Research in Nanotechnology and Science, Indian Institute of Technology Bombay, Mumbai 400076, India.
Department of Physics, Indian Institute of Technology Bombay, Mumbai 400076, India.
ACS Omega. 2021 Jun 29;6(27):17413-17423. doi: 10.1021/acsomega.1c01702. eCollection 2021 Jul 13.
The development of a nanoparticle-based optical platform has been presented as a biosensor for detecting target-specific plant virus DNA. The binding dynamics of gold nanoparticles has been studied on the amine-functionalized surface by the attenuated total reflection (ATR)-based evanescent wave absorption method monitoring the localized surface plasmon resonance (LSPR). The developed surface was established as a refractive index sensor by monitoring the LSPR absorption peak of gold nanoparticles. This nanoparticle-immobilized surface was explored to establish as a biosensing platform with target-specific immunoglobulin (IgG) antibody-antigen interaction. The IgG concentration-dependent variation of absorbance was correlated with the refractive index change. After successfully establishing this ATR configuration as an LSPR-based biosensor, the single-stranded DNA of the chilli leaf curl virus was detected using its complementary DNA sequence as a receptor. The limit of detection of this sensor was determined to be 1.0 μg/mL for this target viral DNA. This ATR absorption technique has enormous potential as an LSPR based nano-biosensor for the detection of other begomoviruses.
一种基于纳米颗粒的光学平台已被开发用作检测靶标特异性植物病毒DNA的生物传感器。通过基于衰减全反射（ATR）的倏逝波吸收法监测局域表面等离子体共振（LSPR），研究了金纳米颗粒在胺功能化表面上的结合动力学。通过监测金纳米颗粒的LSPR吸收峰，将所开发的表面确立为折射率传感器。探索了这种固定有纳米颗粒的表面，以建立一个基于靶标特异性免疫球蛋白（IgG）抗体 - 抗原相互作用的生物传感平台。吸光度随IgG浓度的变化与折射率变化相关。在成功将这种ATR配置确立为基于LSPR的生物传感器后，使用其互补DNA序列作为受体检测了辣椒卷叶病毒的单链DNA。该传感器对这种靶标病毒DNA的检测限确定为1.0μg/mL。作为一种基于LSPR的纳米生物传感器，这种ATR吸收技术在检测其他双生病毒方面具有巨大潜力。
