School of Life Science and Biotechnology, Korea University, Seoul 136-701, Republic of Korea.
FEBS Lett. 2012 Apr 24;586(8):1086-92. doi: 10.1016/j.febslet.2012.03.008. Epub 2012 Mar 13.
Osteoporosis arises from an imbalance between osteoblastic bone formation and osteoclastic bone resorption. In this study, we screened molecules from marine natural products that stimulate osteoblast differentiation. We found that phorbaketal A significantly stimulates osteoblast differentiation in mesenchymal cells. Increased interaction of TAZ and Runx2 stimulated phorbaketal A-induced expression of osteoblastic marker genes. The activation of ERK was important for the stimulation of differentiation because an inhibitor of ERK blocked phorbaketal A-induced osteogenic differentiation. Taken together, the results showed that phorbaketal A stimulates TAZ-mediated osteoblast differentiation through the activation of ERK.
骨质疏松症是由成骨细胞骨形成和破骨细胞骨吸收之间的失衡引起的。在这项研究中，我们从海洋天然产物中筛选出能刺激成骨细胞分化的分子。我们发现，佛波醇酮 A 能显著刺激间充质细胞中的成骨细胞分化。TAZ 和 Runx2 的相互作用增加，刺激了佛波醇酮 A 诱导的成骨细胞标记基因的表达。ERK 的激活对于分化的刺激很重要，因为 ERK 的抑制剂阻断了佛波醇酮 A 诱导的成骨分化。综上所述，结果表明佛波醇酮 A 通过激活 ERK 刺激 TAZ 介导的成骨细胞分化。
