联合给予 α-酮戊二酸（AKG）和 β-羟基-β-甲基丁酸（HMB）对后代骨骼特性产前编程的影响。
Effects of combined maternal administration with alpha-ketoglutarate (AKG) and β-hydroxy-β-methylbutyrate (HMB) on prenatal programming of skeletal properties in the offspring.
机构信息
Department of Animal Physiology, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, ul, Akademicka 12, 20-950, Lublin, Poland.
出版信息
Nutr Metab (Lond). 2012 May 11;9(1):39. doi: 10.1186/1743-7075-9-39.
BACKGROUND
Nutritional manipulations during fetal growth may induce long-term metabolic effects in postnatal life. The aim of the study was to test whether combined treatment of pregnant sows with alpha-ketoglutarate and β-hydroxy-β-methylbutyrate induces additive long-term effects on skeletal system properties in the offspring.
METHODS
The study was performed on 290 pigs obtained from 24 sows divided into 4 equal groups and subjected to experimental treatment during two weeks before delivery. The first group consisted of control sows, while the second group received alpha-ketoglutarate. The third group was treated with β-hydroxy-β-methylbutyrate and the fourth group underwent combined administration of alpha-ketoglutarate and β-hydroxy-β-methylbutyrate. Piglets obtained from sows were reared until slaughter age to perform morphometric, densitometric and mechanical analyses of femur. Serum evaluations of growth hormone, insulin-like growth factor-1, bone-specific alkaline phosphatase and osteocalcin were performed in newborns and 90-day old piglets; additionally, plasma amino acid concentration was measured in newborns.
RESULTS
Maternal treatment with alpha-ketoglutarate and β-hydroxy-β-methylbutyrate significantly reduced fattening time and increased birth body weight, daily body weight gain, bone weight, volumetric bone mineral density, geometrical parameters and mechanical endurance of femur. These effects were associated with increased serum concentrations of growth hormone, insulin-like growth factor-1, bone-specific alkaline phosphatase and osteocalcin. Furthermore, alpha-ketoglutarate and β-hydroxy-β-methylbutyrate administered solely or in combination significantly increased plasma level of 19 amino acids.
CONCLUSIONS
Hormonal and amino acid evaluations in pigs indicate additive effects of AKG and HMB on systemic growth and development; however, determination of bone properties has not shown such phenomenon.
背景
胎儿生长过程中的营养干预可能会在出生后引发长期的代谢效应。本研究旨在测试在怀孕母猪中联合使用α-酮戊二酸和β-羟基-β-甲基丁酸是否会对后代骨骼系统特性产生累加的长期影响。
方法
该研究在 24 头母猪所生的 290 头仔猪上进行，将母猪分为 4 个相等的组，并在分娩前两周进行实验性处理。第一组为对照组母猪，第二组接受α-酮戊二酸处理，第三组接受β-羟基-β-甲基丁酸处理，第四组则接受α-酮戊二酸和β-羟基-β-甲基丁酸的联合给药。从母猪身上获得的仔猪被饲养至屠宰年龄，以对股骨进行形态计量学、密度计量学和力学分析。在新生儿和 90 日龄仔猪中进行生长激素、胰岛素样生长因子-1、骨特异性碱性磷酸酶和骨钙素的血清评估；此外，还在新生儿中测量了血浆氨基酸浓度。
结果
α-酮戊二酸和β-羟基-β-甲基丁酸的母体处理显著缩短了育肥时间，增加了初生体重、日增重、骨重、体积骨密度、几何参数和股骨的力学耐力。这些作用与血清中生长激素、胰岛素样生长因子-1、骨特异性碱性磷酸酶和骨钙素浓度的增加有关。此外，α-酮戊二酸和β-羟基-β-甲基丁酸单独或联合给药均显著增加了 19 种血浆氨基酸的水平。
结论
在猪中的激素和氨基酸评估表明 AKG 和 HMB 对全身生长和发育具有累加作用；然而，对骨特性的测定并未显示出这种现象。
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