Paulus A, Gassmann E, Field M J
Central Analytical Research, Ciba-Geigy, Basel, Switzerland.
Electrophoresis. 1990 Sep;11(9):702-8. doi: 10.1002/elps.1150110906.
Polyacrylamide-filled gel columns are used to separate oligonucleotide samples. For homopolymeric standard samples, plots of migration time versus molecular size are presented over a range of 30-160 bases. With 2.5-4% T and 3.3% C gels, good resolution over the examined mass range, with peak width at half height of 3 to 6 s, is obtained by applying electrical fields of 200-400 V/cm. The separation of heteropolymeric nucleotides by slab gel electrophoresis under routine conditions was compared with capillary gel electrophoresis. Using the same column and the same separation conditions, the plot of migration time versus base number is linear with an identical slope for three oligonucleotide samples which were examined, allowing a calibration of a gel-filled capillary for molecular mass determination.
聚丙烯酰胺填充凝胶柱用于分离寡核苷酸样品。对于同聚物标准样品，在30 - 160个碱基的范围内给出了迁移时间与分子大小的关系图。使用含2.5 - 4% T和3.3% C的凝胶，通过施加200 - 400 V/cm的电场，在检测的质量范围内可获得良好的分辨率，半高峰宽为3至6秒。将常规条件下平板凝胶电泳分离杂聚核苷酸与毛细管凝胶电泳进行了比较。使用相同的柱子和相同的分离条件，对于所检测的三个寡核苷酸样品，迁移时间与碱基数的关系图呈线性，斜率相同，这使得可以对填充凝胶的毛细管进行校准以测定分子量。
