Paulus A, Ohms J I
Research Department, Beckman Instruments, Inc., Palo Alto, CA 94304.
J Chromatogr. 1990 May 16;507:113-23. doi: 10.1016/s0021-9673(01)84187-x.
Analysis of oligonucleotides with gel-filled capillary columns is a fast, efficient and automated way to check their purity. Gel-filled columns were optimized to separate oligonucleotides which were 20-50 nucleotides in length. The influence of sample size on resolution is discussed. A comparison between capillary gel electrophoresis and slab gel electrophoresis in terms of analysis time per sample is made. In addition to homopolymeric samples, the separation of heteropolymeric oligonucleotides with one nucleotide difference in size is presented and suggests the possibility of using capillary gel electrophoresis as a tool for DNA sequencing.
使用填充凝胶的毛细管柱分析寡核苷酸是一种快速、高效且自动化的纯度检测方法。填充凝胶柱经过优化，用于分离长度为20至50个核苷酸的寡核苷酸。讨论了进样量对分离度的影响。对毛细管凝胶电泳和平板凝胶电泳每个样品的分析时间进行了比较。除了均聚物样品外，还展示了对大小相差一个核苷酸的杂聚物寡核苷酸的分离，并表明了使用毛细管凝胶电泳作为DNA测序工具的可能性。
