Yamagishi Kenji, Ike Masakazu, Gau Mitsuru, Tokuyasu Ken
Bioresource Conversion Unit, Food Research Institute, National Agriculture and Food Research Organization.
Japan Agriculture Design Institute.
J Appl Glycosci (1999). 2021 Jul 22;68(3):63-67. doi: 10.5458/jag.jag.JAG-2021_0005. eCollection 2021.
(ER) is greatly appreciated among domestic energy crops in Japan for the production of fermentable sugars from lignocellulosic polysaccharides. In this study, we developed an efficient Ca(OH)-based pretreatment of both stems and leaves of ER at ambient temperature with the addition of a washing step for enzymatic saccharification. The recoveries of glucans and xylans in the pretreated ER after four countercurrent washing cycles were 91 and 76 %, respectively, the former being considerably higher than that of rice straw (RS) (72 %). Their saccharification ratios in the washed sample under the pressure of 1 atm CO were 80 and 92.5 %, respectively. The application of this simple sugar production process from ER would further support the domestic bioprocess development. ER is also foreseen to provide the additional feedstock favorable for harvesting from winter to spring in Japan, preventing a risk for feedstock shortage generated by single harvesting such as RS.
在日本，对于从木质纤维素多糖生产可发酵糖而言，菊苣（ER）在国内能源作物中备受青睐。在本研究中，我们开发了一种基于氢氧化钙的高效预处理方法，在常温下对菊苣的茎和叶进行预处理，并增加了一个洗涤步骤用于酶解糖化。经过四个逆流洗涤循环后，预处理菊苣中葡聚糖和木聚糖的回收率分别为91%和76%，前者显著高于稻草（RS）（72%）。在1个大气压二氧化碳压力下，洗涤后样品中的糖化率分别为80%和92.5%。从菊苣生产简单糖类的这一过程的应用将进一步支持国内生物工艺的发展。预计菊苣还将提供有利于日本冬季至春季收获的额外原料，避免因单一收获如稻草那样造成原料短缺的风险。
