Department of Pharmacology, and Center for Research in Molecular Medicine and Chronic Diseases (CIMUS), Universidade de Santiago de Compostela, Santiago de Compostela, Spain.
J Proteomics. 2012 Dec 5;76 Spec No.:275-86. doi: 10.1016/j.jprot.2012.04.043. Epub 2012 May 8.
Platelets play a fundamental role in hemostasis. Because they do not have a nucleus, proteomics is an ideal way to approach their biochemistry. Platelet proteomics is still a young field that emerged a decade ago. Initial platelet proteomic research focused on general proteome mapping followed by the exploration of sub-cellular compartments, the membrane proteome, and signaling pathways. The initial studies were later completed with the analysis of the platelet releasate and microparticle proteome. The success of these studies led to the application of platelet proteomics to the study of several pathologies where platelets play a fundamental role. Those include platelet-related disorders, such as storage pool disease, gray platelet syndrome, and Quebec platelet disorder; diseases where unwanted platelet activation is highly relevant, such as thrombosis and cardiovascular disease; and other diseases, such as cystic fibrosis, uremia, or Alzheimer's disease. In the present review article, we revise the most relevant proteomic studies on platelet-related diseases carried out to date, paying special attention to sample preparation requirements for platelet clinical proteomic studies. This article is part of a Special Issue entitled: Integrated omics.
血小板在止血中起着至关重要的作用。由于它们没有细胞核，因此蛋白质组学是研究其生物化学的理想方法。血小板蛋白质组学仍然是一个年轻的领域，它在十年前出现。最初的血小板蛋白质组学研究集中在一般蛋白质组图谱绘制上，然后是亚细胞区室、膜蛋白质组和信号通路的探索。最初的研究后来通过对血小板释放物和微粒体蛋白质组的分析完成。这些研究的成功促使血小板蛋白质组学应用于研究血小板起关键作用的几种病理学。这些疾病包括血小板相关疾病，如储存池疾病、灰色血小板综合征和魁北克血小板疾病；与血小板异常激活高度相关的疾病，如血栓形成和心血管疾病；以及其他疾病，如囊性纤维化、尿毒症或阿尔茨海默病。在本文综述中，我们回顾了迄今为止进行的与血小板相关疾病相关的最相关的蛋白质组学研究，特别关注血小板临床蛋白质组学研究的样品制备要求。本文是题为“综合组学”的特刊的一部分。
