Mäler Lena
Department of Biochemistry and Biophysics, The Arrhenius Laboratory, Stockholm University, Stockholm, Sweden.
Mol Membr Biol. 2012 Aug;29(5):155-76. doi: 10.3109/09687688.2012.683456. Epub 2012 May 14.
Many important processes in life take place in or around the cell membranes. Lipids have different properties regarding their membrane-forming capacities, their mobility, shape, size and surface charge, and all of these factors influence the way that proteins and peptides interact with the membrane. In order for us to correctly understand these interactions, we need to be able to study all aspects of the interplay between lipids and peptides and proteins. Solution-state NMR offers a somewhat unique possibility to investigate structure, dynamics and location of proteins and peptides in bilayers. This review focuses on solution NMR as a tool for investigating peptide-lipid interaction, and special attention is given to the various membrane mimetics that are used to model the membrane. Examples from the field of cell-penetrating peptides and their lipid interactions will be given. The importance of studying lipid and peptide dynamics, which reflect on the effect that peptides have on bilayers, is highlighted, and in this respect, also the need for realistic membrane models.
生命中的许多重要过程都发生在细胞膜内或其周围。脂质在形成膜的能力、流动性、形状、大小和表面电荷方面具有不同的特性，所有这些因素都会影响蛋白质和肽与膜相互作用的方式。为了正确理解这些相互作用，我们需要能够研究脂质与肽和蛋白质之间相互作用的各个方面。溶液态核磁共振为研究双层膜中蛋白质和肽的结构、动力学和位置提供了一种 somewhat 独特的可能性。本综述重点关注溶液核磁共振作为研究肽 - 脂质相互作用的工具，并特别关注用于模拟膜的各种膜模拟物。将给出细胞穿透肽领域及其脂质相互作用的实例。强调了研究脂质和肽动力学的重要性，这反映了肽对双层膜的影响，在这方面，也需要现实的膜模型。 （注：原文中“somewhat”直译为“有点”，放在此处语义不太通顺，结合语境可能是想表达“某种程度上”之类的意思，但按照要求未做调整）
