Maeda H, Matsumoto M
Tohoku J Exp Med. 1979 Aug;128(4):313-23.
Effect of a protein antitumor antibiotic, neocarzinostatin (NCS) was examined on Epstein-Barr virus (EBV)-carrying human lymphoid cell lines and normal human lymphocytes. A marked cytotoxic effect of NCS on umbilical cord lymphocytes freshly transformed by EBV was observed, whereas the cells before transformation were affected only very little by NCS. Majority of long-term cultured cell lines were affected only slightly, while shorter-term cultured lines were more susceptible to the effect of NCS. An uptake of FITC-labeled NCS into cells was examined by fluorescence microscopy. The incorporation by the freshly transformed cells was higher than that by the longer-term cultured cell lines. Normal lymphyocytes or heat-killed cells, however, incorporated FITC-NCS very little. On the basis of the known mode of action of NCS on DNA and the present observation, the cytotoxicity of NCS appeared to be exerted within the cells.
研究了蛋白抗肿瘤抗生素新制癌菌素（NCS）对携带爱泼斯坦-巴尔病毒（EBV）的人淋巴样细胞系和正常人淋巴细胞的作用。观察到NCS对刚被EBV转化的脐带淋巴细胞有明显的细胞毒性作用，而转化前的细胞仅受到NCS的极小影响。大多数长期培养的细胞系仅受到轻微影响，而短期培养的细胞系对NCS的作用更敏感。通过荧光显微镜检查了FITC标记的NCS进入细胞的情况。刚转化的细胞对其摄取高于长期培养的细胞系。然而，正常淋巴细胞或热杀死的细胞对FITC-NCS的摄取很少。根据NCS对DNA的已知作用方式和目前的观察结果，NCS的细胞毒性似乎是在细胞内发挥作用的。
