Csako Gyorgy
Department of Laboratory Medicine, Clinical Center, National Institutes of Health, Bethesda, MD, USA.
Methods Mol Biol. 2012;869:147-71. doi: 10.1007/978-1-61779-821-4_13.
Immunofixation electrophoresis (IFE) is a technique for the identification of proteins within complex mixtures after separation by either conventional zone electrophoresis or isoelectric focusing. Most commonly antigens (which are often immunoglobulins) are separated by electrophoresis followed by precipitation with specific antibodies in situ. However, immunoglobulins with specific reactivity can be also precipitated with the proper antigens after electrophoresis in reverse or reversed IFE. Because of its great versatility, potentially high sensitivity, ease to perform and customize, and relatively low cost with no requirement for expensive instrumentation, manual IFE remains a valuable tool for both clinical diagnostic testing and research. Any low-viscosity body fluid specimen or, possibly, culture fluid could be tested with IFE if proper antibodies (or antigens in reverse[d] IFE) are available. After pretreatment with chaotropic and/or reducing agents, even high-viscosity specimens might be amenable to testing with IFE.
免疫固定电泳（IFE）是一种在通过常规区带电泳或等电聚焦分离后鉴定复杂混合物中蛋白质的技术。最常见的是，抗原（通常是免疫球蛋白）通过电泳分离，然后在原位用特异性抗体沉淀。然而，具有特异性反应性的免疫球蛋白在反向或反向IFE电泳后也可以用适当的抗原沉淀。由于其具有广泛的通用性、潜在的高灵敏度、易于操作和定制，以及相对较低的成本且无需昂贵的仪器设备，手动IFE仍然是临床诊断测试和研究的宝贵工具。如果有合适的抗体（或反向IFE中的抗原），任何低粘度体液标本或可能的培养液都可以用IFE进行检测。在用离液剂和/或还原剂进行预处理后，即使是高粘度标本也可能适合用IFE进行检测。
