Bartsch Holger, Arndt Claudia, Koristka Stefanie, Cartellieri Marc, Bachmann Michael
Carl Gustav Carus University TU Dresden, Dresden, Germany.
Methods Mol Biol. 2012;869:481-6. doi: 10.1007/978-1-61779-821-4_42.
Although the main application for polyacrylamide gels is the separation and subsequent blotting of proteins for immunodetection, there are tasks that need staining of proteins in the polyacrylamide gel. Several different staining techniques exist for protein staining in SDS gels that differ in their sensitivity, their expenditure of time, and other aspects. Still, silver staining is the most sensitive and reliable staining technique. Because this technique was developed in the 1970s, a huge number of variations exist. Therefore, we will provide herein three methods, which are robust and easy to perform.
尽管聚丙烯酰胺凝胶的主要应用是分离蛋白质并随后进行免疫检测的印迹，但在聚丙烯酰胺凝胶中仍有对蛋白质进行染色的任务。SDS凝胶中蛋白质染色存在几种不同的染色技术，它们在灵敏度、时间消耗和其他方面存在差异。不过，银染仍是最灵敏和可靠的染色技术。由于该技术是在20世纪70年代开发的，因此存在大量变体。因此，我们在此提供三种方法，它们稳健且易于操作。
