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工程化活性 bc-cbb 型 CIIICIV 超复合体的冷冻电镜结构和复合体之间的电子通讯。

Cryo-EM structures of engineered active bc-cbb type CIIICIV super-complexes and electronic communication between the complexes.

机构信息

Department of Biology, University of Pennsylvania, Philadelphia, PA, USA.

Biochemistry and Molecular Biophysics Graduate Group, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.

出版信息

Nat Commun. 2021 Feb 10;12(1):929. doi: 10.1038/s41467-021-21051-4.

DOI:10.1038/s41467-021-21051-4
PMID:33568648
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7876108/
Abstract

Respiratory electron transport complexes are organized as individual entities or combined as large supercomplexes (SC). Gram-negative bacteria deploy a mitochondrial-like cytochrome (cyt) bc (Complex III, CIII), and may have specific cbb-type cyt c oxidases (Complex IV, CIV) instead of the canonical aa-type CIV. Electron transfer between these complexes is mediated by soluble (c) and membrane-anchored (c) cyts. Here, we report the structure of an engineered bc-cbb type SC (CIIICIV, 5.2 Å resolution) and three conformers of native CIII (3.3 Å resolution). The SC is active in vivo and in vitro, contains all catalytic subunits and cofactors, and two extra transmembrane helices attributed to cyt c and the assembly factor CcoH. The cyt c is integral to SC, its cyt domain is mobile and it conveys electrons to CIV differently than cyt c. The successful production of a native-like functional SC and determination of its structure illustrate the characteristics of membrane-confined and membrane-external respiratory electron transport pathways in Gram-negative bacteria.

摘要

呼吸电子传递复合物以单个实体的形式存在或组合成大型超复合物 (SC)。革兰氏阴性细菌使用类似于线粒体的细胞色素 (cyt) bc (复合物 III，CIII)，并且可能具有特定的 cbb 型细胞色素 c 氧化酶 (复合物 IV，CIV)，而不是典型的 aa 型 CIV。这些复合物之间的电子转移由可溶性 (c) 和膜锚定 (c) 细胞色素介导。在这里，我们报告了一种工程化的 bc-cbb 型 SC (CIIICIV，5.2 Å 分辨率) 和三种天然 CIII 的构象 (3.3 Å 分辨率)。SC 在体内和体外均具有活性，包含所有催化亚基和辅因子，以及两个归因于细胞色素 c 和组装因子 CcoH 的额外跨膜螺旋。细胞色素 c 是 SC 的组成部分，其细胞色素结构域是可移动的，并且它向 CIV 传递电子的方式与细胞色素 c 不同。成功生产出类似天然的功能性 SC 并确定其结构阐明了革兰氏阴性细菌中膜限制和膜外呼吸电子传递途径的特征。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4b3d/7876108/bf9128c6f191/41467_2021_21051_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4b3d/7876108/e485b04c4af1/41467_2021_21051_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4b3d/7876108/945b55a10f97/41467_2021_21051_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4b3d/7876108/1497858eb061/41467_2021_21051_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4b3d/7876108/224758bd7095/41467_2021_21051_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4b3d/7876108/f12d8bef442a/41467_2021_21051_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4b3d/7876108/bf9128c6f191/41467_2021_21051_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4b3d/7876108/e485b04c4af1/41467_2021_21051_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4b3d/7876108/945b55a10f97/41467_2021_21051_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4b3d/7876108/1497858eb061/41467_2021_21051_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4b3d/7876108/224758bd7095/41467_2021_21051_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4b3d/7876108/f12d8bef442a/41467_2021_21051_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4b3d/7876108/bf9128c6f191/41467_2021_21051_Fig6_HTML.jpg

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