凝胶的荧光染色。

Fluorescent staining of gels.

作者信息

Buxbaum Engelbert

机构信息

Ross University School of Medicine, Roseau, Commonwealth of Dominica, West Indies.

出版信息

Methods Mol Biol. 2012;869:543-50. doi: 10.1007/978-1-61779-821-4_48.

DOI:10.1007/978-1-61779-821-4_48

PMID:22585519

Abstract

Certain transition metal complexes show intensive fluorescence when bound to proteins. They can be used to stain gels after electrophoresis with a sensitivity approaching that of silver staining, but in a much simpler and more reproducible procedure. Stains can be prepared easily and at a fraction of the cost of commercially available reagents.Hydrophobic dyes can be used to stain gels without fixing; they do not interfere with later blotting or electro-elution.

摘要

某些过渡金属络合物与蛋白质结合时会发出强烈荧光。它们可用于电泳后对凝胶进行染色，灵敏度接近银染，但操作更简单且可重复性更强。染色剂制备简便，成本仅为市售试剂的一小部分。疏水性染料可用于在不固定的情况下对凝胶进行染色；它们不会干扰后续的印迹或电洗脱操作。