Kurien Biji T, Scofield R Hal
Department of Veterans Affairs, University of Oklahoma Health Sciences Center, Oklahoma Medical Research Foundation, Oklahoma City, OK, USA.
Methods Mol Biol. 2012;869:617-20. doi: 10.1007/978-1-61779-821-4_56.
Several methods have been described to stain proteins analyzed on acrylamide gels. These include ultrasensitive protein detection in one-dimensional and two-dimensional gel electrophoresis using a fluorescent product from the fungus Epicoccum nigrum; a fluorescence-based Coomassie Blue protein staining; visualization of proteins in acrylamide gels using ultraviolet illumination; fluorescence visualization of proteins in sodium dodecyl sulfate-polyacrylamide gels using environmentally benign, nonfixative, saline solution; and increasing the sensitivity four- to sixfold for detecting trace proteins in dye or silver stained polyacrylamide gels using polyethylene glycol 6000. All these methods are reviewed briefly in this chapter.
已经描述了几种对在丙烯酰胺凝胶上分析的蛋白质进行染色的方法。这些方法包括在一维和二维凝胶电泳中使用来自真菌黑附球菌的荧光产物进行超灵敏蛋白质检测；基于荧光的考马斯亮蓝蛋白质染色；利用紫外光照射对丙烯酰胺凝胶中的蛋白质进行可视化；使用环境友好的、非固定的盐溶液对十二烷基硫酸钠 - 聚丙烯酰胺凝胶中的蛋白质进行荧光可视化；以及使用聚乙二醇6000将染料或银染聚丙烯酰胺凝胶中痕量蛋白质的检测灵敏度提高4至6倍。本章将简要回顾所有这些方法。
