Sundaram Ranjini K, Balasubramaniyan Natarajan, Sundaram Pazhani
Recombinant Technologies LLC, Cheshire, CT, USA.
Methods Mol Biol. 2012;869:451-64. doi: 10.1007/978-1-61779-821-4_39.
Staining of proteins separated on gels provides the basis for determination of the critical properties of these biopolymers, such as their molecular weight and/or charge. Detection of proteins on gels and blots require stains. These stains vary in sensitivity, ease of use, color, stability, versatility, and specificity. This review discusses different stains and applications with details on how to use the advantages and disadvantages of each stain. It also compiles some important points to be considered in imaging and evaluation. Commonly used colorimetric and fluorescent dyes for general protein staining, and posttranslational modification-specific detection methods are also discussed.
对凝胶上分离的蛋白质进行染色，为确定这些生物聚合物的关键特性（如分子量和/或电荷）提供了基础。在凝胶和印迹上检测蛋白质需要染色剂。这些染色剂在灵敏度、易用性、颜色、稳定性、通用性和特异性方面各不相同。本综述讨论了不同的染色剂及其应用，并详细介绍了如何利用每种染色剂的优缺点。它还汇总了成像和评估中需要考虑的一些要点。文中还讨论了用于一般蛋白质染色的常用比色染料和荧光染料，以及翻译后修饰特异性检测方法。
