Centre for Emergency Preparedness and Response, Health Protection Agency, Porton Down, Salisbury, UK.
Proteomics. 2012 May;12(9):1431-6. doi: 10.1002/pmic.201100470. Epub 2012 May 14.
Influenza A virus is one of the world's major uncontrolled pathogens, causing seasonal epidemics as well as global pandemics. This was evidenced by the recent emergence and now prevalence of the 2009 swine origin pandemic H1N1 influenza A virus. In this study, quantitative proteomics using stable isotope labelling with amino acids in cell culture was used to investigate the changes in the host cell proteome in cells infected with pandemic H1N1 influenza A virus. The study was conducted in A549 cells that retain properties similar to alveolar cells. Several global pathways were affected, including cell cycle regulation and lipid metabolism, and these could be correlated with recent microarray analyses of cells infected with influenza A virus. Taken together, both quantitative proteomics and transcriptomic approaches can be used to identify potential cellular proteins whose functions in the virus life cycle could be targeted for chemotherapeutic intervention.
甲型流感病毒是世界上主要的未受控病原体之一，可引起季节性流行和全球大流行。最近出现并流行的 2009 年猪源甲型 H1N1 流感病毒就证明了这一点。本研究采用稳定同位素标记的细胞培养氨基酸技术进行定量蛋白质组学研究，以调查感染大流行甲型 H1N1 流感病毒的细胞中宿主细胞蛋白质组的变化。该研究在 A549 细胞中进行，A549 细胞保留了与肺泡细胞相似的特性。多个全球通路受到影响，包括细胞周期调控和脂代谢，这与最近对感染甲型流感病毒的细胞进行的微阵列分析相关。总之，定量蛋白质组学和转录组学方法均可用于鉴定潜在的细胞蛋白，这些蛋白在病毒生命周期中的功能可作为化学治疗干预的靶点。
