Yamaura N, Komagata Y, Saku K, Okubo Y
Department of Microbiology, School of Nursing, Kitasato University.
Kansenshogaku Zasshi. 1990 Oct;64(10):1355-60. doi: 10.11150/kansenshogakuzasshi1970.64.1355.
A monoclonal antibody, SBY1 (IgM, kappa), against the Salmonella O-antigen was generated by using the myeloma cell line Sp2/O-Ag14 as a fusion partner with spleen cells from BALB/c mice immunized with S. senftenberg 963 K. SBY1 was characterized by the slide agglutination and absorption test. SBY1 was believed to show the specificity to O1-, O3- or O19-antigens of Salmonella because S. Senftenberg 963 K (O1, 3, 19) was used as the antigen for immunization. The slide agglutination test with the Salmonella serovars indicated the responsiveness of SBY1. SBY1 was reactive only with strains that possessed O19-antigen. The agglutinating ability of SBY1 was absorbed completely with bacilli possessing O19-antigen. These finding indicates that SBY1 is specific for O19-antigen. Polyclonal factor sera for he serotyping of the O3, 10 group of Salmonella cross-reacted with Salmonella group O1, 3, 19 in the slide agglutination test. In contrast, SBY1 did not cross-react with serovars from several other Salmonella groups. These data suggest the usefulness of SBY1 as a serodiagnostic tool for serotyping of Salmonella.
利用骨髓瘤细胞系Sp2/O-Ag14作为融合伙伴，与用肠炎沙门氏菌963K免疫的BALB/c小鼠的脾细胞融合，产生了一种针对沙门氏菌O抗原的单克隆抗体SBY1（IgM，κ）。通过玻片凝集和吸收试验对SBY1进行了表征。由于使用肠炎沙门氏菌963K（O1、3、19）作为免疫抗原，SBY1被认为对沙门氏菌的O1-、O3-或O-19抗原具有特异性。用沙门氏菌血清型进行的玻片凝集试验表明了SBY1的反应性。SBY1仅与具有O19抗原的菌株发生反应。SBY1的凝集能力被具有O19抗原杆菌完全吸收。这些发现表明SBY1对O19抗原具有特异性。用于沙门氏菌O3、10群血清分型的多克隆因子血清在玻片凝集试验中与O1、3、19群沙门氏菌发生交叉反应。相比之下，SBY1与其他几个沙门氏菌群的血清型不发生交叉反应。这些数据表明SBY1作为沙门氏菌血清分型的血清诊断工具具有实用性。
