UCD School of Mechanical and Materials Engineering, University College Dublin, Belfield, Dublin 4, Ireland.
Biointerphases. 2012 Dec;7(1-4):31. doi: 10.1007/s13758-012-0031-0. Epub 2012 May 3.
Protein adsorption is one of the key parameters influencing the biocompatibility of medical device materials. This study investigates serum protein adsorption and bacterial attachment on polymer coatings deposited using an atmospheric pressure plasma jet system. The adsorption of bovine serum albumin and bovine fibrinogen (Fg) onto siloxane and fluorinated siloxane elastomeric coatings that exhibit water contact angles (θ) ranging from superhydrophilic (θ < 5°) to superhydrophobic (θ > 150°) were investigated. Protein interactions were evaluated in situ under dynamic flow conditions by spectroscopic ellipsometry. Superhydrophilic coatings showed lower levels of protein adsorption when compared with hydrophobic siloxane coatings, where preferential adsorption was shown to occur. Reduced levels of protein adsorption were also observed on fluorinated siloxane copolymer coatings exhibiting hydrophobic wetting behaviour. The lower levels of protein adsorption observed on these surfaces indicated that the presence of fluorocarbon groups have the effect of reducing surface affinity for protein attachment. Analysis of superhydrophobic siloxane and fluorosiloxane surfaces showed minimal indication of protein adsorption. This was confirmed by bacterial attachment studies using a Staphylococcus aureus strain known to bind specifically to Fg, which showed almost no attachment to the superhydrophobic coating after protein adsorption experiments. These results showed the superhydrophobic surfaces to exhibit antimicrobial properties and significantly reduce protein adsorption.
蛋白质吸附是影响医疗器械材料生物相容性的关键参数之一。本研究调查了使用大气压等离子射流系统沉积的聚合物涂层的血清蛋白吸附和细菌附着。研究了水接触角（θ）从超亲水（θ < 5°）到超疏水（θ > 150°）的硅氧烷和氟化硅氧烷弹性体涂层对牛血清白蛋白和牛纤维蛋白原（Fg）的吸附。通过光谱椭圆术在动态流动条件下原位评估蛋白质相互作用。与疏水性硅氧烷涂层相比，超亲水涂层的蛋白质吸附水平较低，优先吸附发生。在表现出疏水性润湿行为的氟化硅氧烷共聚物涂层上也观察到蛋白质吸附水平降低。这些表面上观察到的较低水平的蛋白质吸附表明，氟碳基团的存在具有降低表面对蛋白质附着亲和力的效果。对超疏水硅氧烷和氟硅氧烷表面的分析表明，蛋白质吸附的迹象很少。使用已知特异性结合 Fg 的金黄色葡萄球菌菌株进行的细菌附着研究证实了这一点，在蛋白质吸附实验后，该菌株几乎没有附着在超疏水涂层上。这些结果表明超疏水表面具有抗菌性能，并显著减少蛋白质吸附。
