Zhao Yue, Phillips Brian, Ozcelik Damla, Parks Joshua, Measor Philip, Gulbransen David, Schmidt Holger, Hawkins Aaron R
ECE Department, Brigham Young University, 459 Clyde Building, Provo, UT 84602, USA.
J Biophotonics. 2012 Aug;5(8-9):703-11. doi: 10.1002/jbio.201200049. Epub 2012 May 16.
Liquid filled waveguides that form the basis for on-chip biophotonics diagnostic platforms have primarily found application in fluorescence and Raman spectroscopy experiments that require sensitive discrimination between weak analyte signals and a variety of background signals. Primary sources of background signal can include light from excitation sources (strong, narrow frequency band) and photoluminescence generated in waveguide cladding layers (weak, wide frequency band). Here we review both solid and liquid core filtering structures which are based on anti-resonant reflection that can be integrated with waveguides for attenuating undesirable optical bands. Important criteria to consider for an optimized biosensor include cladding layer materials that minimize broad-spectrum photoluminescence and optimize layer thicknesses for creating a desired spectral response in both solid and liquid guiding layers, and a microfabrication process capable of producing regions with variable spectral response. New results describing how spurious fluorescence can be minimized by optimized thermal growth conditions and how liquid-core filter discrimination can be tuned with liquid core waveguide length are presented.
构成片上生物光子学诊断平台基础的充液波导，主要应用于荧光和拉曼光谱实验，这些实验需要在微弱分析物信号和各种背景信号之间进行灵敏区分。背景信号的主要来源可包括来自激发源的光（强、窄频带）以及在波导包层中产生的光致发光（弱、宽频带）。在此，我们综述基于反谐振反射的固体和液芯滤波结构，它们可与波导集成以衰减不需要的光波段。对于优化的生物传感器而言，需要考虑的重要标准包括：能使广谱光致发光最小化并优化层厚度以在固体和液体引导层中产生所需光谱响应的包层材料，以及能够制造具有可变光谱响应区域的微制造工艺。本文还展示了关于如何通过优化热生长条件使杂散荧光最小化以及如何利用液芯波导长度调节液芯滤波器分辨能力的新结果。
