Universidade Federal de Pernambuco (UFPE), Recife, Brazil.
J Rheumatol. 2012 Jul;39(7):1320-5. doi: 10.3899/jrheum.111027. Epub 2012 May 15.
To analyze the role of interleukin 22 (IL-22) in rheumatoid arthritis (RA).
IL-22 serum levels were measured in 83 patients with established RA under treatment with disease-modifying antirheumatic drugs and in 30 healthy controls matched for age and sex. Patients were assessed for clinical and laboratory variables. Correlations of IL-22 serum levels with disease activity measures [Clinical Disease Activity Index (CDAI) and Disease Activity Score for 28 joints (DAS28)], serological markers, bone erosions, and demographic factors were assessed. Peripheral blood mononuclear cells (PBMC) from 30 patients with RA and 14 controls were purified and stimulated in vitro with phorbol myristate acetate (PMA)/ionomycin. IL-22 production by PBMC and in serum was investigated by ELISA.
IL-22 levels were increased in patients with RA compared with controls (mean 432.37 pg/ml and 67.45 pg/ml, respectively; p < 0.001). Levels of IL-22 correlated with DAS28 and CDAI measures. Rheumatoid factor (RF) positivity was correlated with higher levels of IL-22 in patients with RA (mean 575.08 pg/ml; p = 0.001). The presence of bone erosions was associated with high IL-22 levels (p = 0.0001). PBMC stimulated with PMA/ionomycin expressed higher levels of IL-22 in patients with RA than controls but this was not significant (mean 584.75 pg/ml and 295.57 pg/ml; p = 0.553).
IL-22 is elevated in the serum of patients with established RA. Elevated serum IL-22 allows discrimination between patients with different clinical and laboratory measures and indicates the potential of IL-22 as an additional tool for assessment of activity in RA, particularly in patients with RF antibodies and longterm disease. IL-22 is associated with bone-destructive disease.
分析白细胞介素 22（IL-22）在类风湿关节炎（RA）中的作用。
在接受疾病修饰抗风湿药物治疗的 83 例确诊 RA 患者和 30 名年龄和性别匹配的健康对照者中测量 IL-22 血清水平。评估患者的临床和实验室变量。评估 IL-22 血清水平与疾病活动度测量指标（临床疾病活动指数（CDAI）和 28 个关节疾病活动度评分（DAS28））、血清标志物、骨侵蚀和人口统计学因素的相关性。从 30 例 RA 患者和 14 例对照者中纯化外周血单核细胞（PBMC），并用佛波醇肉豆蔻酸酯（PMA）/离子霉素体外刺激。通过 ELISA 研究 PBMC 和血清中 IL-22 的产生。
与对照组相比，RA 患者的 IL-22 水平升高（分别为 432.37 pg/ml 和 67.45 pg/ml；p < 0.001）。IL-22 水平与 DAS28 和 CDAI 测量值相关。RA 患者的 RF 阳性与更高水平的 IL-22 相关（平均 575.08 pg/ml；p = 0.001）。骨侵蚀的存在与高 IL-22 水平相关（p = 0.0001）。与对照组相比，用 PMA/离子霉素刺激的 RA 患者的 PBMC 表达更高水平的 IL-22，但无统计学意义（分别为 584.75 pg/ml 和 295.57 pg/ml；p = 0.553）。
在确诊 RA 患者的血清中升高了 IL-22。升高的血清 IL-22 可区分不同临床和实验室测量指标的患者，并表明 IL-22 作为 RA 活动评估的附加工具的潜力，尤其是在 RF 抗体和长期疾病患者中。IL-22 与骨破坏性疾病有关。
