Institute for Functional Interfaces-IFG, Karlsruhe Institute of Technology-KIT, Herrmann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, Germany.
Biofouling. 2012;28(5):501-10. doi: 10.1080/08927014.2012.689288.
Conditioning, ie the adsorption of proteins and other macromolecules, is the first process that occurs in the natural environment once a surface is immersed in seawater, but no information is available either regarding the conditioning of surfaces by artificial seawater or whether conditioning affects data obtained from laboratory assays. A range of self-assembled monolayers (SAMs) with different chemical terminations was used to investigate the time-dependent formation of conditioning layers in commercial and self-prepared artificial seawaters. Subsequently, these results were compared with conditioning by solutions in which zoospores of the green alga Ulva linza had been swimming. Spectral ellipsometry and contact angle measurements as well as infrared reflection absorption spectroscopy (IRRAS) were used to reveal the thickness and chemical composition of the conditioning layers. The extent that surface preconditioning affected the settlement of zoospores of U. linza was also investigated. The results showed that in standard spore settlement bioassays (45-60 min), the influence of a molecular conditioning layer is likely to be small, although more substantial effects are possible at longer settlement times.
在自然环境中，一旦表面浸入海水中，就会发生第一个过程，即条件化（conditioning），也就是蛋白质和其他大分子的吸附。但是，关于人工海水中表面的条件化，或者条件化是否会影响从实验室测定中获得的数据，都没有信息。我们使用了一系列具有不同化学末端的自组装单层（SAMs）来研究商用和自制人工海水中条件化层的时变形成过程。随后，将这些结果与绿藻石莼（Ulva linza）游动游孢子的溶液条件化进行了比较。我们采用光谱椭圆测量法和接触角测量法以及红外反射吸收光谱法（IRRAS）来揭示条件化层的厚度和化学组成。我们还研究了表面预处理对石莼游动孢子定居的影响程度。结果表明，在标准孢子定居生物测定（45-60 分钟）中，分子条件化层的影响可能很小，尽管在更长的定居时间内可能会产生更大的影响。
