Nugraha Roni, Finlay John A, Hill Sophie, Fyrner Timmy, Yandi Wetra, Callow Maureen E, Callow James A, Ederth Thomas
a Division of Molecular Physics , IFM, Linköping University , Linköping , Sweden.
Biofouling. 2015;31(1):123-34. doi: 10.1080/08927014.2014.1001841.
The antifouling (AF) properties of oligo(lactose)-based self-assembled monolayers (SAMs), using four different proteins, zoospores of the green alga Ulva linza and cells of the diatom Navicula incerta, were investigated. The SAM-forming alkylthiols, which contained 1, 2 or 3 lactose units, showed significant variation in AF properties, with no differences in wettability. Non-specific adsorption of albumin and pepsin was low on all surfaces. Adsorption of lysozyme and fibrinogen decreased with increasing number of lactose units in the SAM, in agreement with the generally observed phenomenon that thicker hydrated layers provide higher barriers to protein adsorption. Settlement of spores of U. linza followed an opposite trend, being greater on the bulkier, more hydrated SAMs. These SAMs are more ordered for the larger saccharide units, and it is therefore hypothesized that the degree of order, and differences in crystallinity or stiffness between the surfaces, is an important parameter regulating spore settlement on these surfaces.
研究了基于低聚乳糖的自组装单分子层（SAMs）对四种不同蛋白质、绿藻石莼游动孢子和不确定舟形硅藻细胞的防污（AF）性能。形成SAM的烷基硫醇含有1、2或3个乳糖单元，其AF性能存在显著差异，而润湿性无差异。白蛋白和胃蛋白酶在所有表面上的非特异性吸附都很低。溶菌酶和纤维蛋白原的吸附随着SAM中乳糖单元数量的增加而减少，这与普遍观察到的现象一致，即较厚的水合层对蛋白质吸附提供更高的屏障。石莼孢子的沉降呈现相反的趋势，在体积更大、水合程度更高的SAMs上沉降更多。这些SAMs对于较大的糖单元更有序，因此推测表面之间的有序程度以及结晶度或硬度差异是调节这些表面上孢子沉降的重要参数。
