Vater Svenja M, Finlay John, Callow Maureen E, Callow James A, Ederth Thomas, Liedberg Bo, Grunze Michael, Rosenhahn Axel
a Applied Physical Chemistry , Ruprecht-Karls-University Heidelberg , Heidelberg , Germany.
Biofouling. 2015;31(2):229-39. doi: 10.1080/08927014.2015.1022534.
Interaction of zoospores of Ulva linza with cationic, arginine-rich oligopeptide self-assembled monolayers (SAMs) is characterized by rapid settlement. Some spores settle (ie permanently attach) in a 'normal' manner involving the secretion of a permanent adhesive, retraction of the flagella and cell wall formation, whilst others undergo 'pseudosettlement' whereby motile spores are trapped (attached) on the SAM surface without undergoing the normal metamorphosis into a settled spore. Holographic microscopy was used to record videos of swimming zoospores in the vicinity of surfaces with different cationic oligopeptide concentrations to provide time-resolved insights into processes associated with attachment of spores. The data reveal that spore attachment rate increases with increasing cationic peptide content. Accordingly, the decrease in swimming activity in the volume of seawater above the surface accelerated with increasing surface charge. Three-dimensional trajectories of individual swimming spores showed a 'hit and stick' motion pattern, exclusively observed for the arginine-rich peptide SAMs, whereby spores were immediately trapped upon contact with the surface.
石莼游动孢子与富含精氨酸的阳离子寡肽自组装单分子层（SAMs）的相互作用表现为快速沉降。一些孢子以“正常”方式沉降（即永久附着），包括分泌永久性粘合剂、鞭毛缩回和细胞壁形成，而另一些则经历“假沉降”，即游动孢子被困（附着）在SAM表面，而不经历正常的变态成为沉降孢子。全息显微镜用于记录不同阳离子寡肽浓度表面附近游动孢子的视频，以提供与孢子附着相关过程的时间分辨见解。数据显示，孢子附着率随阳离子肽含量的增加而增加。因此，随着表面电荷增加，表面上方海水中游动活性的降低加速。单个游动孢子的三维轨迹显示出“撞击并粘附”的运动模式，这仅在富含精氨酸的肽SAMs中观察到，即孢子与表面接触时立即被困。
