Koizumi O, Mizumoto H, Sugiyama T, Bode H R
Physiological Laboratory, Fukuoka Women's University, Japan.
Neurosci Res Suppl. 1990;13:S165-70. doi: 10.1016/0921-8696(90)90046-6.
Nerve net formation was examined during head-regeneration and budding of Hydra using indirect immunofluorescence on whole mounts. The nerve net was visualized with an antiserum to a neuropeptide, RFamide. The nerve net forms in two steps: the first is the appearance of ganglion cells at the regenerating tip, and the second is the appearance of sensory cells at the apex and the simultaneous disappearance of ganglion cells from the apex. The behavior of epithelial cells during head-regeneration, as defined by monoclonal antibody TS19, corresponded with nerve net formation. We examined nerve net formation in 3 morphogenetic mutants: a head-regeneration deficient mutant (reg-16), a budding deficient mutant (L4) and a multi-headed mutant (mh-1). In addition, we also examined chimeric strains which consist of the epithelial cells from the wild type and nerve cells from a mutant, and vice versa. We obtained clear evidence that nerve net formation is controlled by the environment provided by the epithelial cells.
利用整体标本间接免疫荧光法，在水螅头部再生和出芽过程中研究神经网的形成。用抗神经肽RFamide的抗血清使神经网可视化。神经网的形成分两步：第一步是在再生顶端出现神经节细胞，第二步是在顶端出现感觉细胞，同时顶端的神经节细胞消失。由单克隆抗体TS19定义的上皮细胞在头部再生过程中的行为与神经网形成相对应。我们研究了3种形态发生突变体中的神经网形成：一种头部再生缺陷突变体（reg-16）、一种出芽缺陷突变体（L4）和一种多头突变体（mh-1）。此外，我们还研究了由野生型上皮细胞和突变体神经细胞组成的嵌合菌株，反之亦然。我们获得了明确的证据，证明神经网的形成受上皮细胞提供的环境控制。
