Inoue Masao, Suzuki Satoshi, Akase Zentaro, Shindo Daisuke
JEOL Co., Ltd, Akishima 196-8558, Japan.
J Electron Microsc (Tokyo). 2012 Aug;61(4):217-22. doi: 10.1093/jmicro/dfs046. Epub 2012 May 16.
Simulations were carried out for the orbit of electron-induced secondary electrons around a charged microfibril of a sciatic nerve tissue. In order to set the parameters for the simulation, the shape of the microfibril was determined from a transmission electron microscopy image, while the electric potential on the surface of the charged microfibril was evaluated from a reconstructed phase image obtained with electron holography. On the other hand, the passing point and the angle of secondary electrons at the microfibril surface were determined from a reconstructed amplitude image. Eventually, simulation of orbits of secondary electrons was carried out by changing the kinetic energy of the secondary electrons. Under the given conditions, the orbit of secondary electrons with a kinetic energy of 29.6 eV fits the observations. If there are thin layers of electrons, the secondary electrons do not reach the surface but they go over it due to the repulsive Coulomb force resulting in successive revolving motion around the charged microfibril. Furthermore, the electric field variation due to the movement of the electric charges resulting from the specimen drift is also discussed briefly comparing it with electron holography data.
对坐骨神经组织带电微纤维周围电子诱导二次电子的轨迹进行了模拟。为了设置模拟参数，微纤维的形状由透射电子显微镜图像确定，而带电微纤维表面的电势则由电子全息术获得的重建相位图像评估。另一方面，二次电子在微纤维表面的通过点和角度由重建的振幅图像确定。最终，通过改变二次电子的动能对二次电子的轨迹进行了模拟。在给定条件下，动能为29.6 eV的二次电子轨迹与观测结果相符。如果存在电子薄层，二次电子不会到达表面，而是由于排斥性库仑力从其上方越过，导致围绕带电微纤维连续旋转运动。此外，还简要讨论了由于样品漂移导致电荷移动引起的电场变化，并将其与电子全息术数据进行了比较。
