Rao G N
Abbott Laboratories, Pharmaceutical Products Division, North Chicago, IL 60064.
J Assoc Off Anal Chem. 1987 Sep-Oct;70(5):799-801.
A liquid chromatographic (LC) method is described for the determination of taurine in vitamin and vitamin-mineral premix formulations. The method involves extraction of taurine with 0.1 M bicarbonate buffer, followed by precolumn derivatization with dansyl chloride and LC using fluorescence detection. 6-Aminocaproic acid is used as an internal standard. A reverse phase analytical column and a mobile phase of 0.1 M acetate buffer solution (pH 7.2)-acetonitrile (75 + 25) are used. Vitamins, minerals, and other excipients in the premix formulations do not interfere in the determination. The method is simple, precise, and accurate.
本文描述了一种用于测定维生素和维生素 - 矿物质预混制剂中牛磺酸的液相色谱（LC）方法。该方法包括用0.1 M碳酸氢盐缓冲液萃取牛磺酸，然后用丹磺酰氯进行柱前衍生化，并使用荧光检测进行液相色谱分析。6 - 氨基己酸用作内标。使用反相分析柱和0.1 M醋酸盐缓冲溶液（pH 7.2） - 乙腈（75 + 25）的流动相。预混制剂中的维生素、矿物质和其他辅料不干扰测定。该方法简单、精密且准确。
