Stap J, Aten J A
Laboratory for Radiobiology, University of Amsterdam, The Netherlands.
Strahlenther Onkol. 1990 Nov;166(11):761-3.
The correlation between cell killing and the induction of micro-nuclei was studied for three cell lines after treatment with gamma radiation to investigate whether the frequency of micro-nucleated cells can be used to determine the radiation sensitivity of a cell type. R1 rat rhabdomyosarcoma cells showed a higher sensitivity for the induction of proliferative death than RUC rat ureter carcinoma cells and V79 Chinese hamster cells which had a similar radiation sensitivity. The frequencies of micro-nucleated cells were measured at 48 hours after the treatment. It was determined by time-lapse cinematography that almost all the cells in the treated cultures had divided at that time. Our results indicate that for these cell lines the correlation between the effectiveness for cell killing and the induction of micro-nuclei was the same, within the experimental errors.
为了研究微核诱导与细胞杀伤之间的相关性，用γ射线处理三种细胞系，以探讨微核化细胞的频率是否可用于确定细胞类型的辐射敏感性。R1大鼠横纹肌肉瘤细胞对增殖性死亡诱导的敏感性高于RUC大鼠输尿管癌细胞和V79中国仓鼠细胞，后两者具有相似的辐射敏感性。在处理后48小时测量微核化细胞的频率。通过延时摄影确定，此时处理后的培养物中的几乎所有细胞都已分裂。我们的结果表明，对于这些细胞系，在实验误差范围内，细胞杀伤效力与微核诱导之间的相关性是相同的。
