Choudhry A, Guru P Y, Saxena R P, Tandon A, Saxena K C
Division of Biochemistry, Central Drug Research Institute, Lucknow, India.
Trans R Soc Trop Med Hyg. 1990 May-Jun;84(3):363-6. doi: 10.1016/0035-9203(90)90319-a.
An enzyme-linked immunosorbent assay (ELISA) developed using Leishmania donovani promastigote soluble antigen gave positive responses in practically all the clinically and parasitologically diagnosed patients with kala-azar in a preliminary study. Healthy control subjects from non-endemic and endemic areas gave negative results. No cross-reaction was observed with patients having leprosy, filariasis, malaria, tuberculosis, or amoebiasis. Blood samples collected on filter paper were adequate for the test. The test appears promising in the diagnosis of kala-azar.
使用杜氏利什曼原虫前鞭毛体可溶性抗原开发的酶联免疫吸附测定（ELISA）在一项初步研究中，几乎所有临床和寄生虫学诊断为黑热病的患者中均给出了阳性反应。来自非流行区和流行区的健康对照受试者结果均为阴性。未观察到与麻风病、丝虫病、疟疾、结核病或阿米巴病患者的交叉反应。滤纸上采集的血样足以进行该检测。该检测在黑热病的诊断中似乎很有前景。
