Selivanova G V, Vlasova T D
Tsitologiia. 1990;32(7):704-11.
A study was performed on the human auricle muscle cells which were isolated from biopsies obtained in clinics during operation on heart. The nuclear DNA and the total protein in the cytoplasm were revealed by means of the two consecutive tests: the Feulgen and naphthol yellow S staining. DNA and protein contents were determined by two wave-length scanning cytophotometry. It is ascertained that under mitral defects the investigated parameters exhibit a tendency to increase with age: the nuclear ploidy and total protein content in the cytoplasm rise simultaneously with the increase in nuclear and cellular volumes, the polyploidy reaching higher levels than in myocardium of people of the same age without heart disease. If a patient suffered from ischemia, the nuclear polyploidy increased more slowly than cell hypertrophy to reach the level near the natural age limits.
对从心脏手术临床活检中分离出的人耳廓肌细胞进行了一项研究。通过连续两次检测：福尔根染色和萘酚黄S染色，揭示了细胞核DNA和细胞质中的总蛋白。通过双波长扫描细胞光度法测定DNA和蛋白质含量。已确定，在二尖瓣缺陷情况下，所研究的参数呈现出随年龄增加而增加的趋势：细胞核倍性和细胞质中的总蛋白含量随着细胞核和细胞体积的增加而同时上升，多倍体达到的水平高于同龄无心脏病患者的心肌。如果患者患有缺血，细胞核多倍体的增加比细胞肥大更缓慢，以达到接近自然年龄极限的水平。
