Erokhina I L, Selivanova G V, Vlasova T D, Emel'ianova O I
Tsitologiia. 1995;37(1-2):101-8.
Right atrium myocytes were obtained during open heart surgery from 4 patients with atrial septal defect, and from 1 patient with Fallot's tetrad, aged from 3.5 to 6 years. DNA and total protein contents were measured consecutively in isolated cells using two-wave-length scanning cytophotometry following double staining after Feulgen and with naphthol yellow S. All the biopsies contained polyploid myocytes: the number of 4c cells varied from 18 to 41%, that of 8c cells from 0 to 4%. The average values of cells area and protein content for tetraploid cells were 1.5 times higher that for diploid ones. In all the cases normal and little changed myocytes were dominating (69-91%). The highest number of cells with moderate and severe degenerative changes was registered in the cases of maximum degree of cell hypertrophy and polyploidization.
右心房肌细胞取自4例房间隔缺损患者及1例法洛四联症患者，这些患者均在心脏直视手术过程中获取，年龄在3.5至6岁之间。采用Feulgen染色及萘酚黄S双重染色后，运用双波长扫描细胞光度法对分离出的细胞连续测量DNA和总蛋白含量。所有活检标本均含有多倍体肌细胞：4c细胞数量占比从18%至41%不等，8c细胞数量占比从0至4%不等。四倍体细胞的平均细胞面积和蛋白含量是二倍体细胞的1.5倍。在所有病例中，正常及变化不大的肌细胞占主导（69% - 91%）。在细胞肥大和多倍体化程度最高的病例中，出现中度和重度退行性变的细胞数量最多。
