Thomas C B, Mettler J, Sharp P, Jensen-Kostenbader J, Schultz R D
Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison 53706.
Vet Immunol Immunopathol. 1990 Oct;26(2):143-55. doi: 10.1016/0165-2427(90)90063-x.
The effect of viable Mycoplasma bovis on the in vitro bovine peripheral blood lymphocyte response to phytohemagglutinin (PHA) was studied. Results showed that M. bovis did not act as a mitogen for bovine lymphocytes. Viable M. bovis produced a dose and time dependent suppression of the PHA stimulated lymphocyte response. Suppression was not a result of differences in the viability of infected or control lymphocyte cultures. The suppressive effect of M. bovis was found to be independent of the concentration of PHA used in the test and the lymphocyte response could not be restored by supplementation of the culture medium with arginine. Delay for 48 h after PHA stimulation before adding M. bovis to the lymphocyte cultures diminished, but did not prevent, the suppression of the lymphocyte response. These results show that suppression of the lymphocyte response does not require the presence of M. bovis during the period of PHA stimulation, and that M. bovis was capable of interrupting [3H]-thymidine incorporation in lymphocytes which were actively synthesizing DNA.
研究了活的牛支原体对体外培养的牛外周血淋巴细胞对植物血凝素（PHA）反应的影响。结果表明，牛支原体不是牛淋巴细胞的促有丝分裂原。活的牛支原体对PHA刺激的淋巴细胞反应产生剂量和时间依赖性抑制。抑制作用不是感染或对照淋巴细胞培养物活力差异的结果。发现牛支原体的抑制作用与试验中使用的PHA浓度无关，并且通过在培养基中添加精氨酸不能恢复淋巴细胞反应。在PHA刺激后48小时延迟向淋巴细胞培养物中添加牛支原体可减少但不能阻止淋巴细胞反应的抑制。这些结果表明，淋巴细胞反应的抑制在PHA刺激期间不需要牛支原体的存在，并且牛支原体能够中断正在积极合成DNA的淋巴细胞中[3H] - 胸腺嘧啶核苷的掺入。
