Berka-Zougali Baya, Ferhat Mohamed-Amine, Hassani Aicha, Chemat Farid, Allaf Karim S
Group of Intensification of Transfer Phenomena on Eco-Processes for Industry, Laboratory of Engineering Science for Environment/FRE 3474 CNRS, Pole Science and Technology, University of La Rochelle, Avenue Michel Crepeau, 17042 La Rochelle cedex 01, France.
Laboratory of Research on Bio-active Products and Valorization of Biomasse, Ecole Normale Supérieure, vieux-Kouba 16050, Alger, Algeria.
Int J Mol Sci. 2012;13(4):4673-4695. doi: 10.3390/ijms13044673. Epub 2012 Apr 12.
Two different extraction methods were used for a comparative study of algerian myrtle leaf essential oils: solvent-free-microwave-extraction (SFME) and conventional hydrodistillation (HD). Essential oils analyzed by GC and GC-MS presented 51 components constituting 97.71 and 97.39% of the total oils, respectively. Solvent-Free-Microwave-Extract Essential oils SFME-EO were richer in oxygenated compounds. Their major compounds were 1,8-cineole, followed by α-pinene as against α-pinene, followed by 1,8-cineole for HD. Their antimicrobial activity was investigated on 12 microorganisms. The antioxidant activities were studied with the 2,2-diphenyl-1-picrylhydrazyl (DPPH(•)) radical scavenging method. Generally, both essential oils showed high antimicrobial and weak antioxidant activities. Microstructure analyses were also undertaken on the solid residue of myrtle leaves by Scanning Electronic Microscopy (SEM); it showed that the SFME-cellular structure undergoes significant modifications compared to the conventional HD residual solid. Comparison between hydrodistillation and SFME presented numerous distinctions. Several advantages with SFME were observed: faster kinetics and higher efficiency with similar yields: 0.32% dry basis, in 30 min as against 180 min for HD.
无溶剂微波提取(SFME)和传统水蒸馏(HD)。通过气相色谱(GC)和气相色谱 - 质谱联用(GC - MS)分析的精油分别含有51种成分,占总油含量的97.71%和97.39%。无溶剂微波提取的精油(SFME - EO)富含含氧化合物。其主要成分是1,8 - 桉叶素,其次是α - 蒎烯;而HD提取的精油主要成分是α - 蒎烯,其次是1,8 - 桉叶素。研究了它们对12种微生物的抗菌活性。采用2,2 - 二苯基 - 1 - 苦基肼基(DPPH(•))自由基清除法研究了其抗氧化活性。总体而言,两种精油均表现出高抗菌活性和弱抗氧化活性。还通过扫描电子显微镜(SEM)对香桃木叶的固体残渣进行了微观结构分析;结果表明,与传统HD残渣固体相比,SFME的细胞结构发生了显著变化。水蒸馏和SFME之间存在许多差异。观察到SFME具有几个优点:动力学更快,效率更高,产率相似:干基为0.32%,SFME需30分钟,而HD需180分钟。