Oral Microbiology Laboratory, Istanbul University Faculty of Dentistry, Istanbul, Turkey.
Int J Med Sci. 2012;9(3):256-61. doi: 10.7150/ijms.4382. Epub 2012 May 7.
In this study hypothesing the translocation of oral bacteria from oropharynx into the middle ear cavity may be involved in the pathogenesis of otitis media with effusion (OME), we aimed to investigate the presence and similarity of Fusobacterium nucleatum and Treponema denticola in saliva, nasopharyngeal secretion and the middle ear effusion samples from the children with OME.
Totally 20 children with OME undergoing myringotomy and ventilation tube placement were attended. Stimulated saliva samples were collected after otorhinolaryngological and oral examinations were done. The middle ear effusion and nasopharyngeal secretions were collected during the operations. The presence of F. nucleatum and T. denticola were detected using 16SrRNA-based PCR. The clonal similarities of the bacteria were detected in the samples which the same bacteria had been detected in each samples of the same child. After DNA sequencing, clonal similarity was determined by 16SrRNA gene clone library analysis. The sequences from each clone were compared with similar sequences of reference organisms by FASTA search.
T. denticola was detected only in four (20%) saliva and in one (5%) nasopharyngeal sample. F. nucleatum was detected in 11 (55%) saliva, eight (40%) nasopharyngeal and six (30%) middle ear effusion samples. Sequences from F.nucleatum clones derived from three different anatomic sites within patients were similar in 33% of OME patients, indicating their genetic relatedness.
Bacteria involved in this process most likely originate from the oropharynx since they show a close genetic relatedness with their oropharyngeal counterparts.
本研究假设口腔细菌从咽鼓管迁移到中耳腔可能与分泌性中耳炎（OME）的发病机制有关，我们旨在研究 F. nucleatum 和 T. denticola 在 OME 患儿的唾液、鼻咽分泌物和中耳积液样本中的存在和相似性。
共纳入 20 例接受鼓膜切开术和通气管放置术的 OME 患儿。在进行耳鼻喉科和口腔检查后采集刺激唾液样本。在手术过程中收集中耳积液和鼻咽分泌物。使用基于 16SrRNA 的 PCR 检测 F. nucleatum 和 T. denticola 的存在。在同一患儿的每个样本中均检测到相同细菌的样本中检测细菌的克隆相似性。DNA 测序后，通过 16SrRNA 基因克隆文库分析确定克隆相似性。将每个克隆的序列与参考生物的相似序列通过 FASTA 搜索进行比较。
T. denticola 仅在 4 份（20%）唾液和 1 份（5%）鼻咽样本中检测到。F. nucleatum 在 11 份（55%）唾液、8 份（40%）鼻咽和 6 份（30%）中耳积液样本中检测到。从患者三个不同解剖部位获得的 F.nucleatum 克隆序列在 33%的 OME 患者中相似，表明它们具有遗传相关性。
参与该过程的细菌很可能来自咽鼓管，因为它们与咽鼓管的对应物具有密切的遗传相关性。
