Timby Niklas, Domellöf Magnus, Holgerson Pernilla Lif, West Christina E, Lönnerdal Bo, Hernell Olle, Johansson Ingegerd
Department of Clinical Sciences/Unit of Pediatrics, Umeå University, Umeå, Sweden.
Department of Odontology/Unit of Pedodontics, Umeå University, Umeå, Sweden.
PLoS One. 2017 Jan 18;12(1):e0169831. doi: 10.1371/journal.pone.0169831. eCollection 2017.
In a recent study, supplementation of infant formula with milk fat globule membranes (MFGM) decreased the incidence of otitis media in infants <6 months of age.
The aim of the present study was to characterize the oral microbiota in infants fed MFGM-supplemented formula and compare it to that of infants fed standard formula or breast milk.
In a prospective double-blinded randomized controlled trial, exclusively formula-fed infants <2 months of age were randomized to be fed experimental formula (EF, n = 80) with reduced energy and protein and supplemented with a bovine MFGM concentrate, or standard formula (SF, n = 80) until 6 months of age. A breast-fed reference (BFR, n = 80) group was also recruited. The oral microbiota was analyzed at 4 (n = 124) and 12 (n = 166) months of age using Illumina MiSeq multiplex sequencing and taxonomic resolution against the HOMD 16S rDNA database of oral bacteria.
Species richness in the oral samples did not differ between the EF and SF groups, but partial least square modeling identified a few taxa that were significantly associated with being in either group, e.g. lower level of Moraxella catarrhalis in the EF group. Infants in the BFR group had significantly lower species richness at 4 months of age and their microbiota pattern differed markedly from the formula-fed groups.
Supplementation of infant formula with MFGM yielded moderate effects on the oral microbiome. Moraxella catarrhalis was less prevalent in infants fed EF than in those fed SF and may be associated with the decrease in otitis media seen in the same group.
在最近一项研究中，在婴儿配方奶粉中添加乳脂肪球膜（MFGM）可降低6个月以下婴儿中耳炎的发病率。
本研究旨在描述喂食添加MFGM配方奶粉的婴儿的口腔微生物群特征，并将其与喂食标准配方奶粉或母乳的婴儿的口腔微生物群进行比较。
在一项前瞻性双盲随机对照试验中，将2个月以下纯配方奶粉喂养的婴儿随机分为两组，一组喂食能量和蛋白质含量降低并添加牛MFGM浓缩物的实验配方奶粉（EF，n = 80），另一组喂食标准配方奶粉（SF，n = 80），直至6个月大。还招募了一个母乳喂养参考组（BFR，n = 80）。使用Illumina MiSeq多重测序和针对口腔细菌的HOMD 16S rDNA数据库的分类分辨率，在4个月（n = 124）和12个月（n = 166）时分析口腔微生物群。
EF组和SF组口腔样本中的物种丰富度没有差异，但偏最小二乘模型确定了一些与两组显著相关的分类群，例如EF组中卡他莫拉菌水平较低。BFR组婴儿在4个月时的物种丰富度显著较低，其微生物群模式与配方奶粉喂养组明显不同。
在婴儿配方奶粉中添加MFGM对口腔微生物组有适度影响。喂食EF的婴儿中卡他莫拉菌的流行率低于喂食SF的婴儿，这可能与同一组中中耳炎发病率的降低有关。
