Haraldsson Gunnsteinn, Holbrook W Peter, Könönen Eija
Anaerobe Reference Laboratory, National Public Health Institute (KTL), Mannerheimintie 166, FIN-00300, Helsinki, Finland 2Faculty of Odontology, University of Iceland, Reykjavík, Iceland 3Faculty of Dentistry, Kuwait University, Kuwait.
J Med Microbiol. 2004 Feb;53(Pt 2):161-165. doi: 10.1099/jmm.0.05441-0.
The environment of an infant's nasopharynx during acute otitis media (AOM) favours the growth of anaerobic bacteria, which can be recovered frequently during infection, but hardly at all if the infant is healthy. The aim of this investigation was to identify the potential source and inoculation route of anaerobes that were present in the nasopharynx. Eleven Fusobacterium nucleatum isolates that were collected through the nasal cavity from the nasopharynx of eight infants with a history of AOM, and 161 F. nucleatum isolates from the saliva of the same infants, were typed to the clonal level by using arbitrarily primed PCR (AP-PCR). In five of the eight infants examined, identical AP-PCR types were found among nasopharyngeal and salivary isolates. As anaerobes seem to be present only transiently in the nasopharynx and salivary contamination of the nasopharyngeal samples can be excluded, this observation indicates that the source of nasopharyngeal anaerobes is the oral cavity and that saliva is their transmission vehicle.
急性中耳炎（AOM）期间婴儿鼻咽部的环境有利于厌氧菌生长，感染期间这些厌氧菌常可被检出，但婴儿健康时几乎检测不到。本研究的目的是确定鼻咽部存在的厌氧菌的潜在来源和接种途径。通过鼻腔从8名有AOM病史的婴儿鼻咽部分离出11株具核梭杆菌，并从同一婴儿的唾液中分离出161株具核梭杆菌，采用任意引物PCR（AP-PCR）将其分型至克隆水平。在8名接受检查的婴儿中，有5名婴儿的鼻咽部和唾液分离株中发现了相同的AP-PCR类型。由于厌氧菌似乎仅在鼻咽部短暂存在，且可排除鼻咽部样本的唾液污染，这一观察结果表明鼻咽部厌氧菌的来源是口腔，唾液是其传播媒介。
