School of Life Sciences, Shandong University of Technology, Zibo, China.
Connect Tissue Res. 2012;53(6):508-12. doi: 10.3109/03008207.2012.694928. Epub 2012 Jul 20.
Norcantharidin (NCTD), a chemically modified form of cantharidin, is a potential anticancer drug. In this study, the effects of NCTD on the cellular viability, reactive oxygen species (ROS), mitochondrial membrane potential (MMP), and DNA damage in the human breast cancer cell line Bcap-37 were investigated with confocal and fluorescence microscopy. The cell cycle was further analyzed using the CellQuest software of a Becton-Dickinson FACS flow cytometer. The results indicated that the cellular viability was decreased with the growing concentrations of NCTD and time exposure. Moreover, the fluorescence intensity of ROS was increased, whereas the MMP was decreased in Bcap-37 cells with the growing concentrations of NCTD. NCTD induced a dose-dependent DNA damage and reduced the G1 peak in Bcap-37 cells. The G2/M peak of Bcap-37 was also decreased by the higher concentration of NCTD.
去甲斑蝥素（NCTD）是斑蝥素的化学修饰形式，是一种有潜力的抗癌药物。在这项研究中，应用共聚焦和荧光显微镜观察去甲斑蝥素对人乳腺癌细胞株 Bcap-37 的细胞活力、活性氧（ROS）、线粒体膜电位（MMP）和 DNA 损伤的影响。细胞周期进一步用流式细胞仪的 Becton-Dickinson FACS CellQuest 软件进行分析。结果表明，随着 NCTD 浓度和作用时间的增加，细胞活力逐渐下降。此外，随着 NCTD 浓度的增加，Bcap-37 细胞内 ROS 的荧光强度增加，而 MMP 降低。NCTD 诱导了剂量依赖性的 DNA 损伤，并降低了 Bcap-37 细胞的 G1 峰。较高浓度的 NCTD 也降低了 Bcap-37 细胞的 G2/M 峰。
