Istituto Zooprofilattico Sperimentale Dell'Abruzzo e Del Molise G. Caporale, Teramo, Italy.
Lett Appl Microbiol. 2012 Aug;55(2):122-7. doi: 10.1111/j.1472-765X.2012.03269.x. Epub 2012 Jun 13.
To provide an epidemiologic interpretation of a suspected outbreak of food poisoning caused by Salmonella enterica subspecies enterica serovar Berta strains isolated from humans and from the leftovers of the implicated foods (cream, dairy-based desserts and eggs).
We have correlated the similarity between the strains through genotyping with Pulsed Field Gel Electrophoresis (PFGE), studying antimicrobial sensitivity patterns and epidemiological investigation. The clonal origin of the outbreak was confirmed by all laboratory tests. PFGE analysis of the restriction profiles obtained with XbaI and SpeI revealed a certainly correlation from the strains isolated from the various sources, while the antimicrobial sensitivity pattern was the same in all cases, with all strains sensitive to all antibiotics tested.
Poor hygiene conditions in the facility concerned, lack of hygiene in food handling, high summer temperatures and positive cultures from asymptomatic staff could all be implicated in the infection, with food being the means through which it spread.
This study describes the first outbreak of food poisoning caused by Salmonella enterica subspecies enterica serovar Berta (Salmonella Berta) reported in Italy. It confirms the importance of correlating epidemiological investigations with genotyping and phenotyping to understand the dynamics of infection.
对一起疑似由人类和可疑食物（奶油、含乳制品和鸡蛋）剩余物中分离出的肠炎沙门氏菌亚种肠炎血清型 Berta 菌株引起的食源性中毒爆发进行流行病学解释。
我们通过脉冲场凝胶电泳（PFGE）进行基因分型，研究了抗菌敏感性模式和流行病学调查，对菌株之间的相似性进行了相关性分析。所有实验室检测均证实了暴发的克隆起源。XbaI 和 SpeI 酶切限制图谱的 PFGE 分析显示，从不同来源分离出的菌株之间存在一定的相关性，而所有菌株对抗生素的敏感性模式均相同，所有菌株对所有测试的抗生素均敏感。
相关设施卫生条件差、食品处理过程中卫生状况不佳、夏季高温以及无症状工作人员的阳性培养物都可能与感染有关，而食物是传播的途径。
本研究描述了意大利首次报告的肠炎沙门氏菌亚种肠炎血清型 Berta（肠炎沙门氏菌 Berta）引起的食源性中毒爆发。它证实了将流行病学调查与基因分型和表型分析相关联以了解感染动态的重要性。
