Puohiniemi R, Heiskanen T, Siitonen A
Laboratory of Enteric Pathogens, National Public Health Institute, Helsinki, Finland.
J Clin Microbiol. 1997 Oct;35(10):2487-91. doi: 10.1128/jcm.35.10.2487-2491.1997.
Sprout-borne Salmonella outbreaks in Finland have increased during the last 10 years. The latest two were caused by Salmonella enterica serovar Bovismorbificans (antigenic structure 6,8:r:1,5) in 1994 and S. enterica serovar Stanley (4,5, 12:d:1,2) in 1995. In this study, the restriction fragment length polymorphism of genomic DNA after pulsed-field gel electrophoresis (PFGE) and antimicrobial resistance profiles of the outbreak and nonoutbreak strains were compared. In each separate outbreak, the PFGE patterns of the outbreak strains (40 strains of S. enterica serovar Bovismorbificans and 28 strains of S. enterica serovar Stanley) after digestion of genomic DNA with restriction enzyme XbaI were indistinguishable from each other but differed clearly from those of the nonoutbreak strains (26 strains of S. enterica serovar Bovismorbificans and 40 strains of S. enterica serovar Stanley). The restriction enzyme XhoI did not differentiate the outbreak and nonoutbreak strains. The S. enterica serovar Stanley strains associated with the outbreak also had a unique antimicrobial resistance pattern, whereas all S. enterica serovar Bovismorbificans strains, both outbreak and nonoutbreak strains, were sensitive to all antimicrobial agents tested. Thus, the molecular typing confirmed that the S. enterica serovar Bovismorbificans outbreak isolates from humans and sprout salad were identical and strongly supported the epidemiological finding that S. enterica serovar Stanley outbreak isolates also originated from contaminated alfalfa seeds. It also confirmed that the sources of similar outbreaks in Sweden in 1994 caused by S. enterica serovar Bovismorbificans and in the United States in 1995 caused by S. enterica serovar Stanley and the source of the Finnish outbreaks were common.
在过去10年中，芬兰由豆芽引发的沙门氏菌疫情有所增加。最近的两起疫情分别由1994年的肠炎沙门氏菌牛病亚种（抗原结构6,8:r:1,5）和1995年的肠炎沙门氏菌斯坦利亚种（4,5,12:d:1,2）引起。在本研究中，比较了脉冲场凝胶电泳（PFGE）后基因组DNA的限制性片段长度多态性以及疫情菌株和非疫情菌株的抗菌药物耐药谱。在每一次单独的疫情中，用限制性内切酶XbaI消化基因组DNA后，疫情菌株（40株肠炎沙门氏菌牛病亚种和28株肠炎沙门氏菌斯坦利亚种）的PFGE图谱彼此无法区分，但与非疫情菌株（26株肠炎沙门氏菌牛病亚种和40株肠炎沙门氏菌斯坦利亚种）的图谱明显不同。限制性内切酶XhoI无法区分疫情菌株和非疫情菌株。与疫情相关的肠炎沙门氏菌斯坦利亚种菌株也有独特的抗菌药物耐药模式，而所有肠炎沙门氏菌牛病亚种菌株，无论是疫情菌株还是非疫情菌株，对所有测试的抗菌药物均敏感。因此，分子分型证实，来自人类和豆芽沙拉的肠炎沙门氏菌牛病亚种疫情分离株是相同的，并有力地支持了流行病学研究结果，即肠炎沙门氏菌斯坦利亚种疫情分离株也源自受污染的苜蓿种子。这也证实了1994年瑞典由肠炎沙门氏菌牛病亚种引起的类似疫情以及1995年美国由肠炎沙门氏菌斯坦利亚种引起的类似疫情的源头与芬兰疫情的源头相同。
