Department of Genetics, Stanford University School of Medicine, Stanford, CA, USA.
J Cyst Fibros. 2012 Dec;11(6):502-10. doi: 10.1016/j.jcf.2012.04.008. Epub 2012 May 16.
Fifteen to sixty percent of cystic fibrosis patients harbor Aspergillus fumigatus (Af) in their airways (CF-AC) and some will develop allergic bronchopulmonary aspergillosis (CF-ABPA). Since basophils play a key role in allergy, we hypothesized that they would display alterations in CF-ABPA patients compared to CF-AC or patients without Af colonization (CF).
Using flow cytometry, we measured CD203c, CD63 and CD123 levels on basophils from CF-ABPA (N=11), CF-AC (N=14), and CF (N=12) patients before and after ex vivo stimulation with Af allergens.
Baseline CD203c was increased in basophils from CF-ABPA compared to CF-AC and CF patients. Af extract and recombinant Aspf1 stimulated basophils from CF-ABPA patients to markedly upregulate CD203c, along with modest upregulation of CD63 and a CD123 downward trend. Plasma TARC/CCL17 at baseline and post-stimulation cell supernatant histamine levels were similar in the three groups.
In CF-ABPA, blood basophils are primed and hyperresponsive to Af allergen stimulation.
15%至 60%的囊性纤维化（CF）患者的气道中存在烟曲霉（Af）（CF-AC），其中一些患者将发展为变应性支气管肺曲霉病（CF-ABPA）。由于嗜碱性粒细胞在过敏中起关键作用，我们假设与 CF-AC 或未定植 Af 的 CF 患者（CF）相比，CF-ABPA 患者的嗜碱性粒细胞会发生改变。
通过流式细胞术，我们测量了 CF-ABPA（N=11）、CF-AC（N=14）和 CF（N=12）患者的嗜碱性粒细胞在体外用 Af 过敏原刺激前后 CD203c、CD63 和 CD123 的水平。
CF-ABPA 患者的嗜碱性粒细胞的 CD203c 基线水平高于 CF-AC 和 CF 患者。Af 提取物和重组 Aspf1 刺激 CF-ABPA 患者的嗜碱性粒细胞显著上调 CD203c，同时适度上调 CD63，并呈 CD123 下降趋势。三组患者的基础血浆 TARC/CCL17 和刺激后细胞上清液组胺水平相似。
在 CF-ABPA 中，血液嗜碱性粒细胞被 Af 过敏原刺激致敏并过度反应。
