Eklöf Jens M, Ruda Marcus C, Brumer Harry
Michael Smith Laboratories and Department of Chemistry, University of British Columbia, Vancouver, Canada.
Methods Enzymol. 2012;510:97-120. doi: 10.1016/B978-0-12-415931-0.00006-9.
The ability of β-glucanases to cleave xyloglucans, a family of highly decorated β-glucans ubiquitous in plant biomass, has traditionally been overlooked in functional biochemical studies. An emerging body of data indicates, however, that a spectrum of xyloglucan specificity resides in diverse glycoside hydrolases from a range of carbohydrate-active enzyme families-including classic "cellulase" families. This chapter outlines a series of enzyme kinetic and product analysis methods to establish degrees of xyloglucan specificity and modes of action of glycosidases emerging from enzyme discovery projects.
β-葡聚糖酶切割木葡聚糖（一类在植物生物质中普遍存在的高度修饰的β-葡聚糖）的能力，在传统的功能生化研究中一直被忽视。然而，越来越多的数据表明，一系列木葡聚糖特异性存在于来自多种碳水化合物活性酶家族（包括经典的“纤维素酶”家族）的不同糖苷水解酶中。本章概述了一系列酶动力学和产物分析方法，以确定木葡聚糖特异性程度以及酶发现项目中出现的糖苷酶的作用模式。
