Ghent University, Faculty of Veterinary Medicine, Department of Pathology, Bacteriology and Avian Diseases, Salisburylaan 133, 9820 Merelbeke, Belgium.
Vet J. 2012 Nov;194(2):250-2. doi: 10.1016/j.tvjl.2012.04.011. Epub 2012 May 19.
In pigs, infection with Salmonella Typhimurium often results in the development of carriers that re-excrete the organism during periods of stress. Previous studies have shown that cortisol plays a significant role in the recrudescence of Salmonella Typhimurium and that re-excretion can be induced by injections of dexamethasone. This study evaluated whether a commercially available Salmonella Typhimurium vaccine was able to reduce Salmonella excretion in a model mimicking pre-slaughter stress. Pigs were randomly assigned to either vaccination or a control group and, 5 weeks later, were infected with Salmonella Typhimurium. Twenty-three days post infection, pigs were injected with dexamethasone to induce recrudescence and Salmonella Typhimurium numbers were determined. Salmonella loads were significantly lower in the ileum and colon and in the contents of the ileum and caecum in vaccinated pigs than in non-vaccinated pigs. In addition, significantly more Salmonella positive tonsil and colon samples were found in non-vaccinated pigs. Vaccination with an attenuated vaccine reduced but did not eliminate Salmonella Typhimurium in pigs in conditions mimicking pre-slaughter stress.
在猪中，感染沙门氏菌 Typhimurium 通常会导致携带菌的产生，这些携带菌在应激期间会重新排泄该生物体。先前的研究表明，皮质醇在沙门氏菌 Typhimurium 的复发中起着重要作用，并且可以通过注射地塞米松来诱导重新排泄。本研究评估了一种市售的沙门氏菌 Typhimurium 疫苗是否能够减少模拟宰前应激模型中的沙门氏菌排泄。猪被随机分配到疫苗组或对照组，5 周后感染沙门氏菌 Typhimurium。感染后 23 天，给猪注射地塞米松以诱导复发，并确定沙门氏菌数量。与未接种疫苗的猪相比，接种疫苗的猪的回肠和结肠以及回肠和盲肠内容物中的沙门氏菌负荷明显更低。此外，在未接种疫苗的猪中，更多的扁桃体和结肠样本呈沙门氏菌阳性。在模拟宰前应激条件下，用减毒疫苗接种可减少但不能消除猪中的沙门氏菌 Typhimurium。
