Immunoendocrinology, Division of Medical Biology, Department of Pathology and Medical Biology, University of Groningen, University Medical Center Groningen, Hanzeplein 1, 9700 RB, Groningen, The Netherlands.
Food & Biobased Research, Wageningen University & Research, Bornse Weilanden 9, 6708 WG, Wageningen, The Netherlands.
Sci Rep. 2019 Nov 29;9(1):18017. doi: 10.1038/s41598-019-54353-1.
Routine use of antibiotics in livestock animals strongly contributed to the creation of multidrug-resistant Salmonella Typhimurium strains (STM). Vaccination is an alternative to the use of antibiotics but often suffers from low efficacy. The present study investigated whether long-chain inulin (lcITF) and Lactobacillus acidophilus W37 (LaW37) can support vaccination efficacy against STM and if the interventions influence possible gut microbiota changes. Piglets received daily supplementation until sacrifice. Animals were vaccinated on day 25 after birth, one day after weaning, and were challenged with STM on days 52-54. Dietary intervention with lcITF/LaW37 enhanced vaccination efficacy by 2-fold during challenge and resulted in higher relative abundance of Prevotellaceae and lower relative abundance of Lactobacillaceae in faeces. Although strongest microbial effects were observed post STM challenge on day 55, transient effects of the lcITF/LaW37 intervention were also detected on day 10 after birth, and post-weaning on day 30 where increased relative abundance of faecal lactobacilli was correlated with higher faecal consistency. LcITF treatment increased post-weaning feed efficiency and faecal consistency but did not support vaccination efficacy. Vaccination in immune-immature young animals can be enhanced with functional additives which can simultaneously promote health in an ingredient-dependent fashion.
在牲畜中常规使用抗生素强烈促进了多药耐药性鼠伤寒沙门氏菌（STM）菌株的产生。疫苗接种是抗生素使用的替代方法，但往往效果不佳。本研究探讨了长链菊粉（lcITF）和嗜酸乳杆菌 W37（LaW37）是否可以支持针对 STM 的疫苗接种效果，以及这些干预措施是否会影响可能的肠道微生物群变化。仔猪在处死前每天接受补充。动物在出生后第 25 天、断奶后第 1 天接种疫苗，并在第 52-54 天接受 STM 挑战。lcITF/LaW37 的饮食干预在挑战期间将疫苗接种效果提高了 2 倍，并导致粪便中Prevotellaceae 的相对丰度增加，Lactobacillaceae 的相对丰度降低。尽管在 STM 挑战后的第 55 天观察到最强的微生物效应，但在出生后第 10 天和断奶后第 30 天也检测到 lcITF/LaW37 干预的短暂效应，此时粪便中乳杆菌的相对丰度增加与粪便稠度增加相关。lcITF 处理增加了断奶后的饲料效率和粪便稠度，但不支持疫苗接种效果。在免疫不成熟的幼畜中，疫苗接种可以通过功能性添加剂来增强，这些添加剂可以以成分依赖的方式同时促进健康。
