Hahn Y S, Lenches E M, Galler R, Rice C M, Dalrymple J, Strauss J H
Division of Biology, California Institute of Technology, Pasadena.
Arch Virol. 1990;115(3-4):251-65. doi: 10.1007/BF01310534.
Vaccinia virus recombinants were constructed which contained cDNA sequences encoding the structural region of dengue 2 virus (PR159/S1 strain) or yellow fever virus (17D strain). The flavivirus cDNA sequences were expressed under the control of the vaccinia 7.5k early/late promotor. Cultured cells infected with these recombinants expressed immunologically reactive flavivirus structural proteins, precursor prM and E. These proteins appeared to be cleaved and glycosylated properly since they comigrated with the authentic proteins from dengue 2 virus- and yellow fever virus-infected cells. Mice immunized with the dengue/vaccinia recombinant showed a dengue-specific immune response that included low levels of neutralizing antibodies. Immunization of mice with the yellow fever/vaccinia recombinant was less effective at inducing an immune response to yellow fever virus and in only some of the mice were low titers of neutralizing antibodies produced.
构建了痘苗病毒重组体，其包含编码登革2型病毒（PR159/S1株）或黄热病毒（17D株）结构区域的cDNA序列。黄病毒cDNA序列在痘苗病毒7.5k早期/晚期启动子的控制下表达。用这些重组体感染的培养细胞表达具有免疫反应性的黄病毒结构蛋白、前体prM和E。这些蛋白似乎被正确切割和糖基化，因为它们与来自登革2型病毒和黄热病毒感染细胞的天然蛋白迁移情况相同。用登革热/痘苗重组体免疫的小鼠表现出登革热特异性免疫反应，包括低水平的中和抗体。用黄热/痘苗重组体免疫小鼠诱导对黄热病毒的免疫反应效果较差，只有部分小鼠产生了低滴度的中和抗体。
